Tahara Tatsuki, Koujin Takako, Matsuda Atsushi, Ishii Ayumi, Ito Tomoyoshi, Ichihashi Yasuyuki, Oi Ryutaro
Appl Opt. 2021 Feb 1;60(4):A260-A267. doi: 10.1364/AO.406068.
We present color fluorescence imaging using an incoherent digital holographic technique in which holographic multiplexing of multiple wavelengths is exploited. Self-interference incoherent digital holography with a single-path in-line configuration and the computational coherent superposition scheme are adopted to obtain color holographic three-dimensional information of self-luminous objects with a monochrome image sensor and no mechanical scanning. We perform not only simultaneous color three-dimensional sensing of multiple self-luminous objects but also color fluorescence imaging of stained biological samples. Color fluorescence imaging with an improved point spread function is also demonstrated experimentally by adopting a Fresnel incoherent correlation holography system.
我们展示了一种使用非相干数字全息技术的彩色荧光成像方法，该技术利用了多个波长的全息复用。采用具有单路径同轴配置的自干涉非相干数字全息术和计算相干叠加方案，通过单色图像传感器且无需机械扫描来获取自发光物体的彩色全息三维信息。我们不仅对多个自发光物体进行了同步彩色三维传感，还对染色生物样本进行了彩色荧光成像。通过采用菲涅耳非相干相关全息术系统，还通过实验证明了具有改进点扩散函数的彩色荧光成像。
