Wang Xiao, Zhou Wenxia, Xu Dongdong, Yin Jianhua
J Opt Soc Am A Opt Image Sci Vis. 2021 Mar 1;38(3):337-343. doi: 10.1364/JOSAA.406029.
Based on the polarization property of fluorescent dipoles, fluorescence super-resolution microscopy recently has been proposed by modulating the polarization of the excitation light. In this technique, the super-resolution image is reconstructed by processing the polarization-modulated fluorescence image stack with an iteration algorithm. However, the mechanism of resolution improvement by polarization modulation has been questioned. In this paper, the mechanism of resolution enhancement by polarization modulation is analyzed in reciprocal space. The mathematical model and the reconstruction algorithm of fluorescence super-resolution microscopy via polarization modulation are proposed in reciprocal space. The corresponding simulation results and analysis show that polarization modulation can enlarge the highest detected spatial frequency of fluorescence microscopy to achieve super resolution, which verifies the role of polarization modulation in resolution improvement and provides a useful reference to study fluorescence super-resolution microscopy via polarization modulation in reciprocal space.
基于荧光偶极子的偏振特性，最近有人提出通过调制激发光的偏振来实现荧光超分辨率显微镜成像。在该技术中，通过使用迭代算法处理偏振调制荧光图像堆栈来重建超分辨率图像。然而，偏振调制提高分辨率的机制一直受到质疑。本文在倒易空间中分析了偏振调制提高分辨率的机制。在倒易空间中提出了基于偏振调制的荧光超分辨率显微镜的数学模型和重建算法。相应的仿真结果及分析表明，偏振调制可以扩大荧光显微镜检测到的最高空间频率以实现超分辨率，这验证了偏振调制在提高分辨率方面的作用，并为在倒易空间中研究基于偏振调制的荧光超分辨率显微镜提供了有益的参考。
