Entomology Laboratory of Nova Xavantina, Universidade do Estado de Mato Grosso, Nova Xavantina, Brazil.
Laboratory of Ecology and Conservation, Pós-graduação em Ecologia, Universidade Federal do Pará and Goeldi Museum, Belém, Brazil.
PLoS One. 2021 Mar 9;16(3):e0248216. doi: 10.1371/journal.pone.0248216. eCollection 2021.
Odonata can be sampled following different types of protocols. In Brazil, the most used protocol is the scanning in fixed areas method, where a 100-meter transect is delimited in one of the stream margins, subdivided into 20 segments measuring 5 meters. Despite being universally used, the methodological efficiency or limitations of this protocol for Odonata has never been tested. In this scenario, our objective was to assess the efficiency of the sampling protocol to measure the richness and composition of Odonata in three fundamental aspects: the time of sampling and sampling effort over time and space. We show that the best sampling efficiency was achieved in collections performed at noon, in transects measuring 100 meters, requiring at least two samplings in the same location, supporting the procedures traditionally adopted by many studies with the group. While comparing species composition, we did not see any implication between the different treatments on the capture of the local species pool. However, we highlight and discuss some possible methodological flaws when using this protocol to sample specific Odonata groups. We believe the results obtained are fundamental in the inventory of species and to conduct future studies, as well as to aid conservative measures that use the order Odonata as a tool for environmental monitoring.
蜻蜓可以通过不同的方法进行采样。在巴西，最常用的方法是在固定区域进行扫描，在溪流边缘划定 100 米的样带，分为 20 个 5 米长的段。尽管这种方法被广泛使用，但从未对其在蜻蜓采样方面的方法效率或局限性进行过测试。在这种情况下，我们的目标是评估该采样方案在三个基本方面测量蜻蜓丰富度和组成的效率：采样时间和随时间和空间的采样努力。我们表明，在中午进行的采集，在 100 米的样带中，采样效率最高，至少需要在同一地点进行两次采样，支持了许多研究小组采用的传统程序。在比较物种组成时，我们没有看到不同处理方式对当地物种库的捕获有任何影响。然而，我们强调并讨论了在使用该方案对特定蜻蜓类群进行采样时可能存在的一些方法缺陷。我们认为，获得的结果对于物种清查和未来的研究非常重要，也有助于采取保护措施，将蜻蜓目作为环境监测的工具。
