Afedia S O, Ogunrinade A F
Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.
Vet Parasitol. 1988 Mar;27(3-4):277-81. doi: 10.1016/0304-4017(88)90042-8.
Two techniques for hatching taeniid eggs, based on enzymic proteolysis and disaggregation of the embryophore, were evaluated using Hymenolepis diminuta. Both techniques proved ineffective on H. diminuta in their original form, achieving only a hatching rate of 1-2%. However, a gradual increase in pepsin concentration up to 3 mg ml-1 in the pretreatment solution enhanced hatching significantly (P less than 0.05). Normal or immune rat serum had no appreciable effect on the hatching process.
基于酶促蛋白水解和胚膜解体的两种带绦虫卵孵化技术，使用微小膜壳绦虫进行了评估。两种技术在其原始形式下对微小膜壳绦虫均无效，孵化率仅为1%-2%。然而，预处理溶液中胃蛋白酶浓度逐渐增加至3 mg/ml时，孵化率显著提高（P小于0.05）。正常或免疫大鼠血清对孵化过程没有明显影响。
