Waxham M N, Aronowski J, Server A C, Wolinsky J S, Smith J A, Goodman H M
Department of Neurology, University of Texas Health Science Center, Houston 77225.
Virology. 1988 Jun;164(2):318-25. doi: 10.1016/0042-6822(88)90544-2.
The hemagglutinin-neuraminidase protein (HN) of mumps virus was purified by immunoaffinity chromatography and fragmented by the combined action of CNBr and trypsin. The resulting peptides were separated by HPLC and sequenced by automated Edman degradation. Using this HN-specific amino acid sequence data, a degenerate oligonucleotide was produced and subsequently used to screen a mumps virus cDNA library to isolate HN-specific clones. The complete nucleotide sequence of the HN gene was determined. The monocistronic HN mRNA is approximately 1900 nucleotides long and encodes a single open reading frame of 582 amino acids. The HN protein has a unique hydrophobic stretch of 19 amino acids at its N-terminus that apparently anchors the protein in the viral envelope. A comparison of the mumps virus HN protein sequence with the sequences of the other known paramyxovirus HNs indicates that mumps virus is most closely related to SV-5, followed in decreasing order by NDV, parainfluenza virus 3, and Sendai virus.
通过免疫亲和层析法纯化腮腺炎病毒的血凝素神经氨酸酶蛋白（HN），并通过溴化氰和胰蛋白酶的联合作用将其片段化。所得肽段通过高效液相色谱法分离，并通过自动Edman降解进行测序。利用该HN特异性氨基酸序列数据，制备了简并寡核苷酸，随后用于筛选腮腺炎病毒cDNA文库以分离HN特异性克隆。确定了HN基因的完整核苷酸序列。单顺反子HN mRNA约1900个核苷酸长，编码一个含582个氨基酸的单一开放阅读框。HN蛋白在其N端有一段独特的19个氨基酸的疏水序列，显然将该蛋白锚定在病毒包膜中。将腮腺炎病毒HN蛋白序列与其他已知副粘病毒HN的序列进行比较表明，腮腺炎病毒与SV-5关系最为密切，其次依次为新城疫病毒、副流感病毒3型和仙台病毒。
