凯里酸汤介导大鼠模型中高脂饮食诱导的非酒精性脂肪性肝病减轻的蛋白质组学和脂质组学综合分析

Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model.

作者信息

Cong Shuo, Li Zhengchao, Yu Lei, Liu Yongmei, Hu Yaxin, Bi Ying, Cheng Mingliang

机构信息

School of Basic Medicine Sciences, Guizhou Medical University, Guiyang City, China.

Guizhou Cancer Hospital, Guiyang City, China.

出版信息

Nutr Metab (Lond). 2021 Mar 10;18(1):26. doi: 10.1186/s12986-021-00553-4.

DOI:10.1186/s12986-021-00553-4

PMID:33691721

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7945315/

Abstract

BACKGROUND

Nonalcoholic fatty liver disease (NAFLD) is the most prevalent liver disease and is characterized by excessive fat accumulation. Kaili Sour Soup, a food typical of Guizhou Province, is believed to have significant health benefits. Thus, we aimed to identify and assess the impact of Kaili Sour Soup on NAFLD and its underlying mechanism using integrative proteomic and lipidomic analysis.

METHODS

A high-fat diet and male Wistar rats were used to construct a NAFLD rat model. Haematoxylin and eosin (HE) and Oil Red O staining analyses were used to perform the histologic examination. Proteomic analysis was utilized to systematically identify the global protein profile in NAFLD with and without Kaili Sour Soup treatment. Western blot assays were used to verify the expression of proteins screened by proteomic analysis. Lipidomic analysis was performed to screen lipid metabolism in NAFLD with and without Kaili Sour Soup treatment.

RESULTS

Kaili Sour Soup alleviated high-fat diet (HFD)-induced fatty liver and had a normalizing effect on physiological and biochemical indicators of NAFLD, including body weight, liver weight, liver index, total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and insulin resistance level of homeostasis model assessment (HOMA-IR). Kaili Sour Soup decreased the levels of 13 proteins (Tmem44, Rnaseh2b, Gstm6l, LOC100910877, Rufy4, Slc12a2, Pcif1, P4503A1, Sult1e1, Nop53, AABR07065656.4, AABR07065789.3) that were upregulated by HFD and increased the levels of 3 proteins (Sult1c2, Sult1c2a, Snrnp48) that were downregulated by HFD. Kaili Sour Soup attenuated the HFD-induced increase in acyl carnitine (AcCa) and enhanced the HFD-induced decreases in gangliosides (GM3) and lysophosphatidylserine (LPS) in the NAFLD rat model.

CONCLUSIONS

Altogether, this study revealed that Kaili Sour Soup attenuated HFD-induced fatty liver and systematically identified abnormal proteins and lipids involved in the role of Kaili Sour Soup in a NAFLD rat model.

摘要

背景

非酒精性脂肪性肝病（NAFLD）是最常见的肝脏疾病，其特征是脂肪过度积累。凯里酸汤是贵州省的特色食品，被认为具有显著的健康益处。因此，我们旨在通过蛋白质组学和脂质组学综合分析，确定并评估凯里酸汤对NAFLD的影响及其潜在机制。

方法

采用高脂饮食和雄性Wistar大鼠构建NAFLD大鼠模型。使用苏木精-伊红（HE）染色和油红O染色分析进行组织学检查。利用蛋白质组学分析系统鉴定经凯里酸汤处理和未经处理的NAFLD大鼠的整体蛋白质谱。采用蛋白质免疫印迹法验证蛋白质组学分析筛选出的蛋白质的表达。进行脂质组学分析，以筛选经凯里酸汤处理和未经处理的NAFLD大鼠的脂质代谢情况。

结果

凯里酸汤减轻了高脂饮食（HFD）诱导的脂肪肝，并对NAFLD的生理和生化指标具有归一化作用，包括体重、肝脏重量、肝脏指数、总胆固醇（TC）、甘油三酯（TG）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）以及稳态模型评估（HOMA-IR）的胰岛素抵抗水平。凯里酸汤降低了13种因高脂饮食而上调的蛋白质（Tmem44、Rnaseh2b、Gstm6l、LOC100910877、Rufy4、Slc12a2、Pcif1、P4503A1、Sult1e1、Nop53、AABR07065656.4、AABR07065789.3）的水平，并增加了3种因高脂饮食而下调的蛋白质（Sult1c2、Sult1c2a、Snrnp48）的水平。凯里酸汤减轻了NAFLD大鼠模型中高脂饮食诱导的酰基肉碱（AcCa）增加，并增强了高脂饮食诱导的神经节苷脂（GM3）和溶血磷脂酰丝氨酸（LPS）的减少。