湿磨法结合温度循环策略用于提取对肥胖小鼠肝脂肪变性有缓解作用的蛋白质。

Wet pulverization combined with temperature cycling strategy for extraction of protein with attenuating hepatic steatosis on obese mice.

作者信息

Liu Peng, Gao Chen, Li Wen, Chen Wanchao, Zhang Zhong, Wu Di, Li Tingzhao, Sun Shuai, Yang Yan

机构信息

Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.

University of Shanghai for Science and Technology, Shanghai 200093, China.

出版信息

Food Chem X. 2025 Jan 11;25:102170. doi: 10.1016/j.fochx.2025.102170. eCollection 2025 Jan.

DOI:10.1016/j.fochx.2025.102170

PMID:39897974

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11782863/

Abstract

is an edible fungus with high protein content and excellent bioactivities. However, the effective extraction of . protein has been a challenge, and the amelioration of it against obesity still remain unclear. Herein, . protein (SRA) with mainly molecular weight of 15, 40 and 50 kDa, complete amino acid composition and typical α-helix structure, was obtained by wet pulverization combined with temperature cycling strategy. SRA alleviated the pathological progression in obese mice induced by high-fat diet via regulating lipid metabolism in liver and adipose tissues. SRA could obviously improve hepatic steatosis, which may be attributed to inhibiting MAPK/PGC-1α pathway. Moreover, SRA repaired the cross-talk between gut microbiota and liver via modulating intestinal microecology, and restoring the integrity and function of the intestinal barrier in obese mice. This study highlights an efficient and easy strategy for preparing . protein and its effects against obesity.

摘要

是一种蛋白质含量高且具有优异生物活性的可食用真菌。然而，其蛋白质的有效提取一直是一项挑战，并且其对肥胖的改善作用仍不清楚。在此，通过湿磨结合温度循环策略获得了主要分子量为15、40和50 kDa、氨基酸组成完整且具有典型α-螺旋结构的. 蛋白质（SRA）。SRA通过调节肝脏和脂肪组织中的脂质代谢减轻了高脂饮食诱导的肥胖小鼠的病理进展。SRA可明显改善肝脂肪变性，这可能归因于抑制MAPK/PGC-1α途径。此外，SRA通过调节肠道微生态、恢复肥胖小鼠肠道屏障的完整性和功能来修复肠道微生物群与肝脏之间的相互作用。本研究突出了一种制备. 蛋白质及其抗肥胖作用的高效且简便的策略。