School of Life Sciences, Anhui University, Hefei, Anhui 230601, China.
Anhui Key Laboratory of Modern Biomanufacturing, Hefei, Anhui 230601, China.
J Ind Microbiol Biotechnol. 2021 Apr 30;48(1-2). doi: 10.1093/jimb/kuab013.
The performance of the alkaline fungal laccase PIE5 (pH 8.5) in the delignification and detoxification of alkali-pretreated corncob to produce bioethanol was evaluated and compared with that of the neutral counterpart (rLcc9, 6.5), with the acidic laccase rLacA (4.0) was used as an independent control. Treatment with the three laccases facilitated bioethanol production compared with their respective controls. The lignin contents of alkali-pretreated corncob reduced from 4.06%, 5.06%, and 7.80% to 3.44%, 3.95%, and 5.03%, after PIE5, rLcc9, and rLacA treatment, respectively. However, the performances of the laccases were in the order rLacA > rLcc9 > PIE5 in terms of decreasing total phenol concentration (0.18, 0.36, and 0.67 g/l), boosting ethanol concentration (8.02, 7.51, and 7.31 g/l), and volumetric ethanol productivity (1.34, 0.94, and 0.91 g/l hr), and shortening overall fermentation time. Our results would inform future attempts to improve laccases for ethanol production. Furthermore, based on our data and the fact that additional procedures, such as pH adjustment, are needed during neutral/alkaline fungal laccase treatment, we suggest acidic fungal laccases may be a better choice than neutral/alkaline fungal laccases in bioethanol production.
碱性真菌漆酶 PIE5（pH8.5）在木质素脱除和碱预处理玉米芯解毒以生产生物乙醇中的性能进行了评价，并与中性对照物（rLcc9，6.5）进行了比较，使用酸性漆酶 rLacA（4.0）作为独立对照。与各自的对照物相比，用这三种漆酶处理后，生物乙醇的产量更高。与 rLacA、rLcc9 和 PIE5 处理后木质素含量分别从 4.06%、5.06%和 7.80%降低至 3.44%、3.95%和 5.03%。然而，就降低总酚浓度（0.18、0.36 和 0.67 g/L）、提高乙醇浓度（8.02、7.51 和 7.31 g/L）和提高体积乙醇生产率（1.34、0.94 和 0.91 g/L·hr）以及缩短整体发酵时间而言，漆酶的性能顺序为 rLacA > rLcc9 > PIE5。我们的研究结果将为未来提高漆酶生产乙醇的尝试提供信息。此外，根据我们的数据以及在中性/碱性真菌漆酶处理过程中需要额外的程序（如 pH 调整）的事实，我们建议在生物乙醇生产中，酸性真菌漆酶可能是比中性/碱性真菌漆酶更好的选择。
