Nakakimura K, Sakabe T, Funatsu N, Maekawa T, Takeshita H
Department of Anesthesiology, Yamaguchi University, School of Medicine, Japan.
Anesthesiology. 1988 May;68(5):777-82. doi: 10.1097/00000542-198805000-00017.
The purpose of this study was to examine the effects of enflurane on local cerebral glucose utilization (LCGU), and to provide further insight into the mechanism of the epileptogenic properties of enflurane. Twenty-four male Wistar rats were divided into four groups; three groups with intact cortex received 0.5, 2, or 4% enflurane, and one group with unilateral cortex excised received 4% enflurane. LCGU was measured at each anesthetic concentration using the autoradiographic 2-[14C]deoxyglucose method. LCGU in ten of 33 structures examined during 2% enflurane decreased by 19-33%, and LCGU in 22 structures during 4% enflurane decreased by 19-65%, when compared with that during 0.5% enflurane. While LCGU, in most structures, decreased in a dose-related manner, LCGU in the corpus callosum, thalamic ventrobasal complex, and hippocampal CA3 field during 4% enflurane increased by 31-70%, compared with that during 0.5% and/or 2% enflurane. With unilateral cortical excision during 4% enflurane, the increase in LCGU in the ventrobasal complex was obliterated in the excision side, and the increase in the corpus callosum was attenuated. High LCGU in the hippocampal CA3 field and contralateral ventrobasal complex was not affected with cortical excision. These results indicate that intercortical and corticothalamic pathways are metabolically activated during deep enflurane anesthesia, suggesting that the epileptogenic property of enflurane is related to activation of these pathways.
本研究的目的是检测恩氟烷对局部脑葡萄糖利用（LCGU）的影响，并进一步深入了解恩氟烷致癫痫特性的机制。将24只雄性Wistar大鼠分为四组；三组皮质完整的大鼠分别接受0.5%、2%或4%的恩氟烷，一组单侧皮质切除的大鼠接受4%的恩氟烷。使用放射自显影2-[14C]脱氧葡萄糖法在每个麻醉浓度下测量LCGU。与0.5%恩氟烷时相比，在2%恩氟烷期间检测的33个结构中有10个结构的LCGU下降了19%-33%，在4%恩氟烷期间22个结构的LCGU下降了19%-65%。虽然在大多数结构中LCGU呈剂量依赖性下降，但与0.5%和/或2%恩氟烷时相比，在4%恩氟烷期间胼胝体、丘脑腹侧基底复合体和海马CA3区的LCGU增加了31%-70%。在4%恩氟烷期间进行单侧皮质切除时，切除侧腹侧基底复合体中LCGU的增加消失，胼胝体中的增加减弱。海马CA3区和对侧腹侧基底复合体中较高的LCGU不受皮质切除的影响。这些结果表明，在深度恩氟烷麻醉期间皮质间和皮质丘脑通路在代谢上被激活，提示恩氟烷的致癫痫特性与这些通路的激活有关。
