Levashova Z B, Morozova T M
Biokhimiia. 1988 Feb;53(2):251-5.
It was demonstrated that cell cytosol and nuclei of estradiol-dependent mammary tumours and kidney of the rat contain a polyamine-dependent protein kinase whose activity is stimulated 2-5 fold by 2 mM spermine and is inhibited by heparin (8-10 micrograms/ml). This protein kinase uses acid proteins (casein) as substrates and ATP and GTP as phosphate sources. The polyamine-dependent protein kinase is eluted from DEAE-cellulose with 0.22-0.24 M NaCl. In cell nuclei the enzyme activity is 3-5 times as high as that in the cytoplasm. In cell nuclei of hormone-dependent tumours and kidney estradiol increases the activity of the polyamine-dependent protein kinase 2-5 times within 30 minutes.
已证明，大鼠依赖雌二醇的乳腺肿瘤细胞胞质溶胶和细胞核以及肾脏中含有一种多胺依赖性蛋白激酶，其活性在2 mM精胺作用下可被刺激2至5倍，并被肝素（8 - 10微克/毫升）抑制。这种蛋白激酶以酸性蛋白（酪蛋白）为底物，以ATP和GTP为磷酸源。多胺依赖性蛋白激酶用0.22 - 0.24 M NaCl从DEAE - 纤维素上洗脱下来。在细胞核中，该酶活性比细胞质中的高3至5倍。在激素依赖性肿瘤和肾脏的细胞核中，雌二醇在30分钟内可使多胺依赖性蛋白激酶的活性增加2至5倍。
