College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China.
Chem Commun (Camb). 2021 Apr 14;57(29):3555-3558. doi: 10.1039/d1cc00168j. Epub 2021 Mar 11.
A donor-cell-assisted membrane biotinylation strategy was used to modify small extracellular vesicles (sEVs) while minimizing protein damage, and allowed the sEVs to be loaded onto carriers. Biotinylated programmed death-ligand 1 (PD-L1) positive sEVs were used to select for aptamers from a DNA library. PD-L1 negative sEVs from a homologous cell line were found to remove non-specific aptamer sequences to increase the specificity. After just four rounds, high-affinity aptamers for PD-L1 positive sEVs were selected as novel affinity reagents.
采用供体细胞辅助的膜生物素化策略来修饰小细胞外囊泡 (sEVs)，同时最大限度地减少蛋白质损伤，并使 sEVs 加载到载体上。生物素化的程序性死亡配体 1 (PD-L1) 阳性 sEVs 用于从 DNA 文库中筛选适体。从同源细胞系中分离到 PD-L1 阴性 sEVs，以去除非特异性适体序列，从而提高特异性。仅经过四轮筛选，就选择出了对 PD-L1 阳性 sEVs 具有高亲和力的适体作为新型亲和试剂。
