Department of Biology, Faculty of Science, University of Guilan, Namjoo St. Faculty of Science, Rasht, 4193833697, Iran.
Caspian Sea Basin Research Center, Department of Marine Sciences, University of Guilan, Rasht, Iran.
Bull Environ Contam Toxicol. 2021 May;106(5):773-778. doi: 10.1007/s00128-021-03164-0. Epub 2021 Mar 11.
The aquatic environment is constantly exposed to chemical pollutants from agriculture and the urban environment. In this study, the effects of Bisphenol A, Naphthalene, and Butachlor on metabolic and antioxidant enzymes of goldfish were investigated during subacute exposure. The samples (n = 144, 100.97 ± 31.47 g) were distributed randomly in 12 glass aquaria (70 L) with a stocking density of 12 fish for each. Each aquarium was exposed to one of each pollutant (Bisphenol A with 500 μg/L, Butachlor with 0.28 μL/L and Naphthalene with 200 μg/L) separately, while the control group remained without contaminants for the entire 5 weeks of the experiment. The result showed that the activity of AST (Aspartate transaminase), ALT (Alanine transaminase), ALP (alkaline phosphatases), and POD (Guaiacol Peroxidase) significantly altered in contaminants treatments. The POD level, an antioxidant enzyme, showed a significant increase, especially on the 14th day of exposure to Bisphenol A (p < 0.05). In conclusion, these enzymes, as a biomarker, can be useful in environmental biomonitoring of aquatic ecosystems.
水生环境不断受到农业和城市环境中化学污染物的影响。在这项研究中，在亚急性暴露期间，研究了双酚 A、萘和丁草胺对金鱼代谢和抗氧化酶的影响。将样本（n=144，100.97±31.47g）随机分配到 12 个玻璃水族箱（70 升）中，每个水族箱中饲养 12 条鱼。每个水族箱分别暴露于一种污染物（双酚 A 500μg/L、丁草胺 0.28μL/L 和萘 200μg/L），而对照组在整个 5 周的实验期间保持无污染物状态。结果表明，AST（天冬氨酸转氨酶）、ALT（丙氨酸转氨酶）、ALP（碱性磷酸酶）和 POD（愈创木酚过氧化物酶）的活性在污染物处理中明显改变。抗氧化酶 POD 水平显著升高，特别是在暴露于双酚 A 的第 14 天（p<0.05）。总之，这些酶作为生物标志物，可以用于水生生态系统的环境生物监测。
