一种基于荧光的测定活黑色素瘤细胞中葡萄糖摄取的方法。
A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells.
作者信息
Grahovac Jelena, Pavlović Marijana, Ostojić Marija
机构信息
Experimental Oncology Department, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.
出版信息
Methods Mol Biol. 2021;2265:73-80. doi: 10.1007/978-1-0716-1205-7_5.
PMID:33704706
Abstract
Melanoma cells have high glycolytic capacity. Glucose uptake is a key rate-limiting step in glucose utilization. Here we describe a simple protocol for measuring direct glucose uptake in living melanoma cells by flow cytometry.
摘要
黑色素瘤细胞具有较高的糖酵解能力。葡萄糖摄取是葡萄糖利用中的关键限速步骤。在此，我们描述一种通过流式细胞术测量活的黑色素瘤细胞中直接葡萄糖摄取的简单方法。
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