Wang Tao, Kaufman Russel E
Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, USA.
The Wistar Institute, Philadelphia, PA, USA.
Methods Mol Biol. 2021;2265:119-128. doi: 10.1007/978-1-0716-1205-7_9.
Tumor-associated macrophages (TAMs) are one of most important components of the tumor microenvironment. Although many assays have been developed to differentiate monocytes into macrophages (Mϕ) for studying the biology of TAMs in vitro, little is known whether the macrophages induced by these approaches can recapitulate the biology of TAMs present in the tumor microenvironment. We have developed a novel assay to differentiate human monocytes into TAMs using modified melanoma-conditioned medium, which is derived from the concentrated tumor cell culture medium. Characterization of these modified melanoma-conditioned medium-induced macrophages (MCMI-Mϕ) by multiple flow cytometry, Luminex, microarray, and immunohistochemistry analyses indicates that MCMI-Mϕ are phenotypically and functionally highly similar to the TAMs present in the tumor microenvironment.
肿瘤相关巨噬细胞（TAM）是肿瘤微环境最重要的组成部分之一。尽管已经开发了许多检测方法来将单核细胞分化为巨噬细胞（Mϕ），以便在体外研究TAM的生物学特性，但对于这些方法诱导产生的巨噬细胞是否能够重现肿瘤微环境中TAM的生物学特性，人们了解甚少。我们开发了一种新型检测方法，利用改良的黑色素瘤条件培养基将人单核细胞分化为TAM，该培养基源自浓缩的肿瘤细胞培养基。通过多种流式细胞术、Luminex、微阵列和免疫组织化学分析对这些改良的黑色素瘤条件培养基诱导的巨噬细胞（MCMI-Mϕ）进行表征，结果表明MCMI-Mϕ在表型和功能上与肿瘤微环境中存在的TAM高度相似。
