Siruvallur Murali Vasanth, Cobanoglu Murat Can, Welf Erik S
Lyda Hill Department of Bioinformatics and Department of Cell Biolog, UT Southwestern Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2021;2265:155-171. doi: 10.1007/978-1-0716-1205-7_12.
Researchers often aim to incorporate microenvironmental variables such as the dimensionality and composition of the extracellular matrix into their cell-based assays. A technical challenge created by introduction of these variables is quantification of single-cell measurements and control of environmental reproducibility. Here, we detail a methodology to quantify viability and proliferation of melanoma cells in 3D collagen-based culture platforms by automated microscopy and 3D image analysis to yield robust, high-throughput results of single-cell responses to drug treatment.
研究人员通常旨在将细胞外基质的维度和组成等微环境变量纳入基于细胞的检测中。引入这些变量所带来的一个技术挑战是单细胞测量的量化以及环境可重复性的控制。在此，我们详细介绍一种方法，通过自动显微镜和三维图像分析来量化黑色素瘤细胞在基于三维胶原蛋白的培养平台中的活力和增殖情况，从而得出针对药物治疗的单细胞反应的可靠、高通量结果。
