Istituto Superiore di Sanità, Rome, Italy.
Methods Mol Biol. 2021;2291:1-17. doi: 10.1007/978-1-0716-1339-9_1.
Shiga toxin-producing Escherichia coli (STEC) are human pathogens causing severe diseases, such as hemorrhagic colitis and the hemolytic uremic syndrome. The prompt diagnosis of STEC infection is of primary importance to drive the most appropriate patient's management procedures. The methods to diagnose STEC infections include both direct isolation of the STEC from stool samples and the identification of indirect evidences based on molecular, phenotypic, and serological applications. Here, the procedures in use at the Italian Reference Laboratory for E. coli infections are described.
产志贺毒素大肠杆菌（STEC）是人类病原体，可引起严重疾病，如出血性结肠炎和溶血尿毒综合征。及时诊断 STEC 感染对于推动最适当的患者管理程序至关重要。诊断 STEC 感染的方法包括直接从粪便样本中分离 STEC 和基于分子、表型和血清学应用的间接证据的鉴定。本文描述了意大利大肠杆菌感染参考实验室使用的程序。
