Jang Jae Cheol, Kim Dong Hyuk, Jang Young Dal, Kim Yoo Yong
Department of Agricultural Biotechnology, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Department of Animal and Food Science, University of Wisconsin-River Falls, River Falls, WI 54022, USA.
Anim Biosci. 2021 Dec;34(12):1974-1980. doi: 10.5713/ab.21.0019. Epub 2021 Mar 11.
The objective of this study was to estimate standardized ileal digestibility (SID) of amino acids (AA) in growing pigs fed diets containing increasing levels of copra meal (CM) with β-mannanase supplementation.
Twenty barrows (initial body weight: 34.43±0.11 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in metabolism crates. Pigs were allotted to 5 dietary treatments in a completely randomized design with 4 replicates per treatment. The dietary treatments were: i) NC, negative control, corn-soybean meal (SBM) based diet, ii) PC, positive control, basal diet + 0.10% β-mannanase supplementation (800 IU/kg), iii) CM6, PC diet with 6% CM supplementation, iv) CM12, PC diet with 12% CM supplementation, and v) CM18, PC diet with 18% CM supplementation. A nitrogen-free diet was used to estimate basal endogenous losses of AA for SID calculation. All experimental diets contained 0.5% chromic oxide as an indigestible marker. Each period consisted of a 4-d diet adaptation period and a 3-d ileal digesta collection period.
There were no differences in apparent ileal digestibility (AID) and SID of all AA between the NC and PC treatments except that the PC treatment had lower AID and SID of glycine than the NC treatment (p<0.05). There were linear decreases in AID and SID of lysine (p<0.05) and aspartic acid (p = 0.06; tendency) with increasing levels of CM in the diets with β-mannanase.
The β-mannanase supplementation had no effect on AA digestibility in pigs fed the corn-SBM based diet but increasing levels of CM reduced SID of lysine and aspartic acid.
本研究旨在评估在含不同水平椰粕(CM)且添加β-甘露聚糖酶的日粮喂养下生长猪的氨基酸(AA)标准化回肠消化率(SID)。
20头去势公猪(初始体重:34.43±0.11千克)在回肠末端手术安装T型插管,单独饲养于代谢笼中。采用完全随机设计将猪分为5种日粮处理组,每组4个重复。日粮处理如下:i)NC,阴性对照,以玉米-豆粕(SBM)为基础的日粮;ii)PC,阳性对照,基础日粮+0.10%β-甘露聚糖酶添加量(800 IU/kg);iii)CM6,PC日粮+6%CM添加量;iv)CM12,PC日粮+12%CM添加量;v)CM18,PC日粮+18%CM添加量。使用无氮日粮来估计用于SID计算的AA基础内源损失。所有实验日粮均含有0.5%的氧化铬作为不可消化标记物。每个阶段包括4天的日粮适应期和3天的回肠食糜收集期。
除了PC处理组的甘氨酸表观回肠消化率(AID)和SID低于NC处理组(p<0.05)外,NC和PC处理组之间所有AA的AID和SID没有差异。在添加β-甘露聚糖酶的日粮中,随着CM水平的增加,赖氨酸(p<0.05)和天冬氨酸(p = 0.06;趋势)的AID和SID呈线性下降。
添加β-甘露聚糖酶对以玉米-SBM为基础日粮喂养的猪的AA消化率没有影响,但CM水平的增加会降低赖氨酸和天冬氨酸的SID。