Altered postnatal developmental patterns of ultrasonic vocalizations in Dock4 knockout mice.

Ultrasonic vocalization (USV) characterization is useful for evaluating communication in mouse models of autism spectrum disorder (ASD). Here, by categorizing USVs into 12 types using a comprehensive classification method, we obtained the qualitative and quantitative characteristics of USV repertoire emitted by ASD-related Dock4 knockout (KO) mice and their wild-type (WT) littermates during social isolation over early postnatal development. Notably, USVs emitted by WT pups exhibited a developmental switch from a pattern with more multiple-note calls, which have more complex acoustic structure, lower pitch and larger volume, into one with more single-note calls, which have simpler acoustic structure, higher pitch and smaller volume. Comparing with WT pups, USVs emitted by Dock4 KO pups had larger volume and consisted of more multiple-note calls with higher pitch in later developmental stage. These findings collectively reveal a developmental pattern of USV in normal mice and identified a set of alterations in Dock4 KO pups.