Department of Integrative and Systems Physiology, Faculty of Medical Sciences, and Life Science Innovation Center, University of Fukui, Fukui 910-1193, Japan.
Department of Integrative and Systems Physiology, Faculty of Medical Sciences, and Life Science Innovation Center, University of Fukui, Fukui 910-1193, Japan.
Cell Calcium. 2021 Jun;96:102386. doi: 10.1016/j.ceca.2021.102386. Epub 2021 Mar 4.
NCLX was identified as a mitochondrial Na-Ca exchanger. However, contribution of NCLX to overall mitochondrial Na-Ca exchange activity remains unclear, especially in brain mitochondria where plasma membrane Na-Ca exchanger NCX also exists. We studied the issue using isolated mouse brain mitochondria. The Na- as well as Li-dependent Ca efflux from mitochondria was significantly inhibited by a NCLX blocker, but was insensitive to NCX blockers, suggesting that NCLX comprises a major part in forward mode of mitochondrial Na-Ca exchange activity. On the other hand, the Na-dependent Ca influx into mitochondria, the reverse mode, was insensitive to all the blockers tested, suggesting unidentified Ca transport systems.
NCLX 被鉴定为一种线粒体钠钙交换器。然而，NCLX 对整体线粒体钠钙交换活性的贡献仍不清楚，特别是在脑线粒体中，也存在质膜钠钙交换器 NCX。我们使用分离的小鼠脑线粒体研究了这个问题。NCLX 阻断剂显著抑制了线粒体的钠依赖和锂依赖的钙外流，但对 NCX 阻断剂不敏感，这表明 NCLX 构成了线粒体钠钙交换活动正向模式的主要部分。另一方面，对所有测试的阻断剂均不敏感的钠依赖的钙内流，即反向模式，表明存在未鉴定的钙转运系统。
