林氏亨氏海星（海星纲：棘海星科）卵母细胞中核糖体基因的扩增及染色体外核仁的形成。

Amplification of ribosomal genes and formation of extrachromosomal nucleoli in oocytes of starfish Henricia hayashi (Asteroidea: Echinasteridae).

作者信息

Gaginskaya E R, Kasyanov V L, Kogan G L

机构信息

Laboratory of Electron Microscopy and Comparative Histology, State University of Leningrad, U.S.S.R.

出版信息

Cell Differ. 1988 Mar;23(1-2):53-60. doi: 10.1016/0045-6039(88)90036-x.

DOI:10.1016/0045-6039(88)90036-x

PMID:3370678

Abstract

DNA and RNA specific dyes, Ag-NOR staining and in situ hybridization were used for studying the nucleolar apparatus in the growing oocytes of Henricia hayashi (Asteroidea: Echinasteridae). A plasmid containing ribosomal genes of Drosophila melanogaster (Kolchinsky et al., 1980) labelled with 3H by nick-translation served as an rDNA probe. Multiple extrachromosomal nucleoli are formed by the cascade type as a result of growth and subsequent fragmentation of the chromosomal (primary) rDNA body and its derivative extrachromosomal (secondary) rDNA bodies. Ribosomal genes were shown in all nucleolar structures. Argentophilia of the primary and secondary DNA bodies appears to be due to the dense packing of the rDNA-containing material. Ag(+) NORs were detected in the extrachromosomal multiple nucleoli and NOR complexes. Amplification of rDNA is a highly probable conclusion from the existing data.

摘要

采用DNA和RNA特异性染料、银染核仁组织区（Ag-NOR）染色及原位杂交技术，对林氏亨丽海星（海百合纲：棘海星科）生长中的卵母细胞的核仁器进行研究。用缺口平移法标记有3H的含有黑腹果蝇核糖体基因的质粒（科尔钦斯基等人，1980年）作为rDNA探针。由于染色体（初级）rDNA体及其衍生的染色体外（次级）rDNA体的生长和随后的片段化，多个染色体外核仁以级联方式形成。在所有核仁结构中均显示有核糖体基因。初级和次级DNA体的嗜银性似乎是由于含rDNA物质的紧密堆积所致。在染色体外多个核仁和NOR复合体中检测到Ag(+) NORs。根据现有数据，rDNA扩增是极有可能的结论。