在非变性条件下，通过凝胶过滤高效液相色谱法从精液中一步高产率纯化人前列腺酸性磷酸酶。
One-step, high-yield purification of human prostatic acid phosphatase from seminal fluid by gel-filtration HPLC under nondenaturing conditions.
作者信息
Rusciano D, Agostini E, Ceccarini C, Terrana B
机构信息
Cellular Biology Laboratory, Sclavo Research Center, Siena, Italy.
出版信息
Clin Chem. 1988 May;34(5):984-6.
PMID:3370802
Abstract
This is a fast, efficient method for purification to homogeneity of human prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2], from seminal fluid. Use of a "high-pressure" liquid-chromatographic gel-filtration column permits high-yield recovery of the purified enzyme with most of its enzymatic and immunological activity retained.
摘要
这是一种从精液中纯化人前列腺酸性磷酸酶[正磷酸单酯磷酸水解酶（最适酸性），EC 3.1.3.2]至均一性的快速、高效方法。使用“高压”液相色谱凝胶过滤柱可实现纯化酶的高收率回收，且大部分酶活性和免疫活性得以保留。
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