维生素D受体、尾相关同源盒2基因多态性与癌症风险的可能关联。

Possible association of Vitamin D receptor, caudal-related homeobox 2 polymorphism with the risk of cancer.

作者信息

Rehman Madiha, Mahboob Tabassum, Shahid Syed M

机构信息

Department of Biochemistry, University of Karachi, Karachi, Sindh, Pakistan.

School of Health Science, Eastern Institute of Technology, Auckland, New Zealand.

出版信息

Int J Health Sci (Qassim). 2021 Mar-Apr;15(2):9-13.

PMID:33708039

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7934131/

Abstract

OBJECTIVE

This study was conducted to find out the possible association of Vitamin D receptor, caudal-related homeobox 2 (VDR-Cdx2) polymorphism with cancer in the given study group.

METHODS

In this study, 151 subjects (84 cases and 67 controls) were recruited from two local tertiary care hospitals of Karachi, Pakistan, suffering from various cancers including gastric cancer (GC), rectal cancer (RC), colon cancer (CC), and multiple myeloma followed by ethical approval from institutions and informed consent from all the participants. The genotyping of VDR-Cdx2 polymorphism was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction method. The genotypic assortment/distribution in the control and disease groups was according to Hardy-Weinberg's equilibrium.

RESULTS

The genotype frequencies of VDR-Cdx2 polymorphism in cancer patients were observed as: AA 1.2%, AG 32%, and GG 66.8% while in control group as; AA 7.5%, AG 50.7%, and GG was 41.8%. The results unveil that the genotype VDR-Cdx2 was found significantly different in cancer and control group ( < 0.01). The AG and GG genotypes were found to be associated with the cancer ( < 0.05). Therefore, these genotypes may be considered as the risk factors for cancer. However, the frequencies of "A" and "G" alleles were not significantly different between two groups.

CONCLUSION

The observed single-nucleotide polymorphism of VDR-Cdx2 gene may be considered as a risk factor for the cancer in this study group. The AG and GG genotypes established an association with various cancers including GC, RC, CC, and multiple myeloma in Pakistani population. Further investigations examining large data are required to compare the role of VDR-Cdx2 polymorphism in cancer etiology in related population.

摘要

目的

本研究旨在找出给定研究组中维生素D受体-尾相关同源盒2（VDR-Cdx2）基因多态性与癌症之间可能存在的关联。

方法

本研究从巴基斯坦卡拉奇的两家当地三级护理医院招募了151名受试者（84例病例和67名对照），他们患有包括胃癌（GC）、直肠癌（RC）、结肠癌（CC）和多发性骨髓瘤在内的各种癌症，研究获得了机构的伦理批准，并取得了所有参与者的知情同意。采用四引物扩增阻滞突变系统-聚合酶链反应法对VDR-Cdx2基因多态性进行基因分型。对照组和疾病组的基因型分类/分布符合哈迪-温伯格平衡。

结果

癌症患者中VDR-Cdx2基因多态性的基因型频率观察为：AA 1.2%、AG 32%、GG 66.8%，而对照组为：AA 7.5%、AG 50.7%、GG 41.8%。结果表明，VDR-Cdx2基因型在癌症组和对照组中存在显著差异（P<0.01）。发现AG和GG基因型与癌症相关（P<0.05）。因此，这些基因型可能被视为癌症的危险因素。然而，两组之间“A”和“G”等位基因的频率没有显著差异。