人结肠癌中免疫淋巴细胞与浆细胞样树突状细胞的相关性
Correlation Between Immune Lymphoid Cells and Plasmacytoid Dendritic Cells in Human Colon Cancer.
作者信息
Wu Jing, Cheng Hang, Wang Helei, Zang Guoxia, Qi Lingli, Lv Xinping, Liu Chunyan, Zhu Shan, Zhang Mingyou, Cui Jiuwei, Ueno Hideki, Liu Yong-Jun, Suo Jian, Chen Jingtao
机构信息
Institute of Translational Medicine, The First Hospital, Jilin University, Changchun, China.
Department of Pediatrics, The First Hospital, Jilin University, Changchun, China.
出版信息
Front Immunol. 2021 Feb 23;12:601611. doi: 10.3389/fimmu.2021.601611. eCollection 2021.
BACKGROUND
Innate lymphoid cells (ILCs), so far studied mostly in mouse models, are important tissue-resident innate immune cells that play important roles in the colorectal cancer microenvironment and maintain mucosal tissue homeostasis. Plasmacytoid dendritic cells (pDCs) present complexity in various tumor types and are correlated with poor prognosis. pDCs can promote HIV-1-induced group 3 ILC (ILC3) depletion through the CD95 pathway. However, the role of ILC3s in human colon cancer and their correlation with other immune cells, especially pDCs, remain unclear.
METHODS
We characterized ILCs and pDCs in the tumor microenvironment of 58 colon cancer patients by flow cytometry and selected three patients for RNA sequencing.
RESULTS
ILC3s were negatively correlated, and pDCs were positively correlated, with cancer pathological stage. There was a negative correlation between the numbers of ILC3s and pDCs in tumor tissues. RNA sequencing confirmed the correlations between ILC3s and pDCs and highlighted the potential function of many ILC- and pDC-associated differentially expressed genes in the regulation of tumor immunity. pDCs can induce apoptosis of ILC3s through the CD95 pathway in the tumor-like microenvironment.
CONCLUSIONS
One of the interactions between ILC3s and pDCs is the CD95 pathway, which may help explain the role of ILC3s in colon cancer.
背景
天然淋巴细胞（ILC）目前大多在小鼠模型中进行研究，是重要的组织驻留天然免疫细胞，在结直肠癌微环境中发挥重要作用并维持黏膜组织稳态。浆细胞样树突状细胞（pDC）在各种肿瘤类型中表现出复杂性，且与预后不良相关。pDC可通过CD95途径促进HIV-1诱导的3型天然淋巴细胞（ILC3）耗竭。然而，ILC3在人类结肠癌中的作用及其与其他免疫细胞，尤其是pDC的相关性仍不清楚。
方法
我们通过流式细胞术对58例结肠癌患者肿瘤微环境中的ILC和pDC进行了表征，并选择3例患者进行RNA测序。
结果
ILC3与癌症病理分期呈负相关，pDC与癌症病理分期呈正相关。肿瘤组织中ILC3和pDC的数量呈负相关。RNA测序证实了ILC3和pDC之间的相关性，并突出了许多与ILC和pDC相关的差异表达基因在肿瘤免疫调节中的潜在功能。在肿瘤样微环境中，pDC可通过CD95途径诱导ILC3凋亡。
结论
ILC3和pDC之间的相互作用之一是CD95途径，这可能有助于解释ILC3在结肠癌中的作用。
Zotero 插件