Diomede L, Masturzo P, Agosti S, Ornaghi F, Salmona M
Laboratory for Enzyme Research, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Clin Chim Acta. 1988 Mar 15;172(2-3):161-9. doi: 10.1016/0009-8981(88)90320-8.
An electrochemical method is described for the determination of lecithin in rat and human amniotic fluid. Choline is released from lecithin enzymatically by phospholipase D and the hydrogen peroxide released by the action of choline oxidase is quantitatively determined by peroxidase-catalyzed rupture of the covalent C-F bond of 4-fluorophenol. The concentration of F- ions in solutions is determined by a fluoride sensitive electrode from the resulting cell potential difference recorded before and 10 min after addition of a solution containing phospholipase D, choline oxidase and horseradish peroxidase. Lecithin levels in rat amniotic fluid increased from about 10 mumol/l on the 20th day of gestation to 80 mumol/l on day 21, which corresponds to the time of spontaneous delivery. In human amniotic fluid the lecithin concentrations determined with this new method parallel those already reported. They were approximately 10 to 50 mumol/l between the 15th and 18th weeks of gestation and increased from 5- to 7-fold between the 37th and 41st weeks of pregnancy. This method was only slightly influenced by the presence of blood or meconium contamination in the amniotic fluid.
本文描述了一种用于测定大鼠和人类羊水卵磷脂的电化学方法。卵磷脂经磷脂酶D酶解释放出胆碱，胆碱氧化酶作用释放的过氧化氢通过过氧化物酶催化断裂4-氟苯酚的共价C-F键进行定量测定。通过氟离子敏感电极根据加入含磷脂酶D、胆碱氧化酶和辣根过氧化物酶溶液之前及之后10分钟记录的电池电位差来测定溶液中F-离子的浓度。大鼠羊水卵磷脂水平在妊娠第20天约为10 μmol/L，到第21天增加至80 μmol/L，这与自然分娩时间相对应。用这种新方法测定的人类羊水卵磷脂浓度与已报道的结果相符。妊娠第15至18周时约为10至50 μmol/L，妊娠第37至41周时增加了5至7倍。该方法受羊水中血液或胎粪污染的影响较小。
