K Venkatakrishna, Hv Sudeep, K Shyamprasad
R&D Center for Excellence, Vidya Herbs Pvt Ltd, Bangalore, India.
SAGE Open Med. 2021 Feb 24;9:2050312120984885. doi: 10.1177/2050312120984885. eCollection 2021.
Despite having numerous physiological benefits, toxicological assessment of green coffee beans is sparce. Here, we document the oral acute and sub-chronic toxicity of a standardized decaffeinated green coffee bean extract containing 50% chlorogenic acids (CGA-7™) in rats.
We have performed a limit test at single oral dose of 2000 mg/kg to evaluate the acute toxicity in female Wistar rats. Furthermore, repeated dose 90-day toxicity study was conducted to assess the risk of long-term use of CGA-7.
A 14-day observation revealed no clinical signs of toxicity or mortality in animals at 2000 mg/kg acute oral dose of CGA-7. The administration of 250, 500, and 1000 mg/kg CGA-7 showed significant alterations in some parameters such as food consumption, relative organ weights of brain and spleen, haematological and biochemical parameters compared to control. These changes were not consistent and dose-dependent throughout the study. Furthermore, the changes were within the physiological range and toxicologically insignificant. CGA-7 did not affect the normal metabolism and physiology of the animals up to 1000 mg/kg dose. Macroscopic and histological examination of organs did not reveal any organ toxicity.
Finally, the findings from this study suggest the safety of green coffee bean extract.
尽管生咖啡豆具有多种生理益处，但对其毒理学评估却很少。在此，我们记录了一种含有50%绿原酸（CGA-7™）的标准化脱咖啡因生咖啡豆提取物对大鼠的经口急性和亚慢性毒性。
我们以2000mg/kg的单次经口剂量进行了极限试验，以评估对雌性Wistar大鼠的急性毒性。此外，还进行了重复剂量90天毒性研究，以评估长期使用CGA-7的风险。
14天的观察显示，在2000mg/kg的CGA-7急性经口剂量下，动物没有出现毒性的临床症状或死亡。与对照组相比，给予250、500和1000mg/kg的CGA-7后，在一些参数上出现了显著变化，如食物消耗量、脑和脾的相对器官重量、血液学和生化参数。在整个研究过程中，这些变化并不一致且不呈剂量依赖性。此外，这些变化在生理范围内，毒理学意义不大。在高达1000mg/kg的剂量下，CGA-7并未影响动物的正常代谢和生理功能。对器官的大体和组织学检查未发现任何器官毒性。
最后，本研究结果表明生咖啡豆提取物是安全的。
