Bailyes E M, Seymour P M, Fulton I, Price C P, Luzio J P
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, UK.
Clin Chim Acta. 1988 Mar 15;172(2-3):267-74. doi: 10.1016/0009-8981(88)90333-6.
Intestinal ALP was purified from meconium to a specific activity of 1,100 U/mg protein and used as antigen in the preparation of 7 monoclonal antibodies. Two of these antibodies were specific for intestinal ALP and reacted with different epitopes. Both bound adult intestinal ALP better than fetal intestinal ALP. One of these antibodies was used to establish a capture assay for intestinal ALP in human serum over the range 0.5-16 U/l. Reference ranges of serum intestinal ALP concentrations were established in relation to blood groups. Measurement of intestinal ALP in the serum of pregnant women showed no correlation with pre-term fetal passage of meconium.
从胎粪中纯化出肠碱性磷酸酶，其比活性达到1100 U/mg蛋白质，并用作抗原制备7种单克隆抗体。其中两种抗体对肠碱性磷酸酶具有特异性，且与不同表位发生反应。二者与成人肠碱性磷酸酶的结合能力均优于胎儿肠碱性磷酸酶。其中一种抗体被用于建立一种捕获检测法，用于检测人血清中0.5 - 16 U/l范围内的肠碱性磷酸酶。根据血型建立了血清肠碱性磷酸酶浓度的参考范围。对孕妇血清中肠碱性磷酸酶的检测表明，其与胎粪早产无相关性。
