Du Xing, Li Qiqi, Yang Liu, Zeng Qiang, Wang Siqi, Li Qifa
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Front Cell Dev Biol. 2021 Feb 23;9:610553. doi: 10.3389/fcell.2021.610553. eCollection 2021.
, the first lincRNA associated with sow fertility, has been shown to control granulosa cell (GC) functions and follicular atresia. However, the underlying mechanism is not fully understood. In this study, RNA-seq was performed and we noticed that inhibition of led to dramatic transcriptomic alterations in porcine GCs. A total of 1,272 differentially expressed transcripts were identified, including 1167 DEmRNAs and 105 DEmiRNAs. Furthermore, protein-protein interaction, gene-pathway function, and TF-miRNA-mRNA regulatory networks were established and yielded four regulatory modules with multiple hub genes, such as , , , , , , , , and . Functional assessment showed that these hub DEGs were mainly enriched in TGF-β, PI3K-Akt, FoxO, Wnt, MAPK, and ubiquitin pathways that are essential for GC states (apoptosis and proliferation) and functions (hormone secretion). , we also found that knockdown of in porcine GCs significantly induced cell apoptosis, impaired cell viability, and suppressed 17β-estradiol (E2) synthesis. Notably, four candidate genes for sow reproductive traits (, , β, and ) were also identified as potential targets of . These findings present a panoramic view of the transcriptome in -reduced GCs, highlighting that , a candidate lincRNA for sow fertility, is crucial for the normal states and functions of GCs.
[具体名称]，首个与母猪繁殖力相关的长链非编码RNA，已被证明可调控颗粒细胞（GC）功能和卵泡闭锁。然而，其潜在机制尚未完全明确。在本研究中，我们进行了RNA测序，发现抑制[具体名称]会导致猪颗粒细胞发生显著的转录组改变。共鉴定出1272个差异表达转录本，包括1167个差异表达mRNA和105个差异表达miRNA。此外，我们构建了蛋白质-蛋白质相互作用、基因-通路功能以及转录因子- miRNA - mRNA调控网络，并产生了四个具有多个枢纽基因的调控模块，如[具体基因1]、[具体基因2]、[具体基因3]、[具体基因4]、[具体基因5]、[具体基因6]、[具体基因7]、[具体基因8]和[具体基因9]。功能评估表明，这些枢纽差异表达基因主要富集于TGF-β、PI3K - Akt、FoxO、Wnt、MAPK和泛素通路，这些通路对于颗粒细胞的状态（凋亡和增殖）及功能（激素分泌）至关重要。此外，我们还发现，在猪颗粒细胞中敲低[具体名称]会显著诱导细胞凋亡、损害细胞活力并抑制17β-雌二醇（E2）合成。值得注意的是，四个母猪繁殖性状的候选基因（[具体基因10]、[具体基因11]、β和[具体基因12]）也被鉴定为[具体名称]的潜在靶点。这些发现展示了[具体名称]减少的颗粒细胞转录组的全景，突出了作为母猪繁殖力候选长链非编码RNA的[具体名称]对于颗粒细胞的正常状态和功能至关重要。
