Laboratory of Animal Health (LARSANA), Federal University of Western Pará (UFOPA), Santarém, Brazil.
Department of Clinical Science, College of Veterinary Medicine and Animal Science, University of Sao Paulo (FMVZ/USP), São Paulo, Brazil.
J Vet Emerg Crit Care (San Antonio). 2021 Mar;31(2):269-273. doi: 10.1111/vec.13053. Epub 2021 Mar 12.
The objective of this study was to evaluate the biochemical and blood gas alterations of whole blood of buffaloes that was stored in citrate-phosphate-dextrose with adenine (CPDA-1) and CPD/SAG-M blood bags for 42 days.
Prospective study.
Ten male buffaloes were used in this study. A total volume of 900 mL of blood was collected from each buffalo so that 450 mL was stored in CPDA-1 and 450 mL was stored in CPD/SAG-M bags at 2-6°C for 42 days. The stored blood was evaluated at 7 time points (D): D0 (immediately after blood collection) and 7 (D7), 14 (D14), 21 (D21), 28 (D28), 35 (D35), and 42 (D42) days after collection. Blood gas, biochemical, and microbiological parameters were monitored.
The overall blood pH decreased from 6.997 ± 0.05 at D0 to 6.784 ± 0.09 at D42, differing from baseline from D14 onward (P < 0.05). There were increases in partial pressure of oxygen (pO ), partial pressure of carbon dioxide (pCO ), lactate, and potassium (K) and decreases in the concentrations of sodium, bicarbonate, glucose, and pH (P < 0.05) during storage in both bags but no alterations in total protein concentration. Most of the variables were consistently similar between the 2 types of blood bags (P > 0.05) evaluated, with the exception of pCO , HCO cholesterol, and total protein, which had higher values in the CPDA-1 bag (P < 0.05). The K, pO , and lactate had the highest alterations during storage, with increases from baseline to D42 of 563%, 317%, and 169%, respectively.
In general, no significant changes of clinical importance were observed after storage of whole blood samples from buffaloes for 42 days in the 2 types of blood bags that are indicated for use with this species.
本研究旨在评估在枸橼酸-磷酸盐-葡萄糖-腺嘌呤（CPDA-1）和 CPD/SAG-M 血袋中储存 42 天的水牛全血的生化和血气变化。
前瞻性研究。
本研究使用了 10 头雄性水牛。从每头水牛采集 900ml 的全血，其中 450ml 储存在 CPDA-1 中，450ml 储存在 CPD/SAG-M 袋中，温度为 2-6°C，储存 42 天。在采集后 7 个时间点（D）评估储存的血液：D0（采集后立即）和 7（D7）、14（D14）、21（D21）、28（D28）、35（D35）和 42（D42）天。监测血气、生化和微生物学参数。
总体血液 pH 值从 D0 时的 6.997 ± 0.05 下降到 D42 时的 6.784 ± 0.09，从 D14 开始与基线不同（P < 0.05）。在两个袋子中储存期间，氧分压（pO2）、二氧化碳分压（pCO2）、乳酸和钾（K）增加，而钠、碳酸氢盐、葡萄糖和 pH 值浓度降低（P < 0.05），但总蛋白浓度没有变化。除了 CPDA-1 袋中 pCO2、HCO3-、胆固醇和总蛋白值较高外（P < 0.05），两种类型的血袋（P > 0.05）评估的大多数变量均保持相似。K、pO2 和乳酸在储存期间变化最大，与基线相比，分别增加了 563%、317%和 169%。
一般来说，在两种适用于该物种的血袋中储存水牛全血样本 42 天后，未观察到具有临床重要意义的显著变化。
