Faculty of Agronomy and Veterinary Medicine, University of Brasília, Brasilia, Brazil.
Large Animal Veterinary Teaching Hospital, University of Brasília, Brasilia, Brazil.
Vet Clin Pathol. 2021 Jun;50(2):221-226. doi: 10.1111/vcp.12973. Epub 2021 Apr 25.
Anaerobic cellular metabolism causes a series of structural and physiologic changes during storage that could compromise post-transfusion viability, reducing the safety of using blood stored for an extended period.
We aimed to follow the biochemical and hematologic alterations of equine blood stored in plastic bags containing citrate-phosphate-dextrose-adenine (CPDA-1) for up to 28 days.
Whole blood samples (450 mL) were collected from 20 Brazilian Saddle horses into CPDA-1 pouches and stored between 2°C and 6°C in a blood bank. On days 0, 7, 14, 21, and 28 of storage, blood samples were taken and submitted for biochemical (sodium [Na ], potassium [K ], glucose, and lactate) and hematologic (hemoglobin [Hb], hematocrit [HCT], mean corpuscular volume [MCV], percent hemolysis [% hemolysis]) analyses.
The only time the blood pH levels dipped below 7 was after D21 of storage, and the levels were significantly lower than those on the first storage day (D0). Potassium concentrations showed significant increases from D7 and then remained increased throughout the experimental period. Chloride and lactate concentrations revealed a significantly increased trend from D7 that was maintained over time. Mean corpuscular volumes increased significantly on D7 and D14 and, thereafter, remained stable. The mean % hemolysis increased on D28, which was significantly higher than D0. No bacterial growth was found in any pouch after 28 days of storage.
Significant and gradual biochemical changes were observed in equine whole blood during prolonged storage. These changes could compromise the clinical conditions of patients requiring transfusion. In vivo studies are needed to evaluate the effects as well as survival rates and efficacy of transfused red blood cells in recipients.
在储存过程中，无氧细胞代谢会引起一系列结构和生理变化，这可能会损害输注后的活力，降低使用储存时间延长的血液的安全性。
我们旨在观察在含有柠檬酸盐-磷酸盐-葡萄糖-腺嘌呤（CPDA-1）的塑料袋中储存长达 28 天的马血的生化和血液学变化。
从 20 匹巴西鞍马中采集 450 毫升全血样本到 CPDA-1 袋中，并在血液银行中 2°C 至 6°C 之间储存。在储存的第 0、7、14、21 和 28 天，采集血样进行生化（钠[Na]、钾[K]、葡萄糖和乳酸）和血液学（血红蛋白[Hb]、红细胞压积[HCT]、平均红细胞体积[MCV]、溶血百分比[%溶血]）分析。
只有在储存第 21 天之后，血液 pH 值才降至 7 以下，且水平明显低于第一天（D0）。从 D7 开始，钾浓度显著增加，然后在整个实验期间一直保持增加。从 D7 开始，氯和乳酸浓度呈显著增加趋势，并随时间保持不变。D7 和 D14 时平均红细胞体积显著增加，此后保持稳定。D28 时平均溶血百分比增加，明显高于 D0。在 28 天的储存后，没有在任何一个袋子中发现细菌生长。
在长时间储存过程中，马全血发生了显著且逐渐的生化变化。这些变化可能会损害需要输血的患者的临床状况。需要进行体内研究来评估输注的红细胞在受者中的影响、存活率和疗效。
