Qi Ming, Gao Shufeng, Nie Sihui, Wang Ke, Guo Lingling
Ultrasound Department, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Functional Division, Jinzhou Yixian People's Hospital, Jinzhou, China.
Drug Deliv. 2021 Dec;28(1):569-579. doi: 10.1080/10717544.2021.1889721.
We report the formulation of nanoassemblies (NAs) comprising C225 conjugates Gd-PFH-NAs (C-Gd-PFH-NAs) for low-intensity focused ultrasound diagnosis ablation of thyroid cancer. C-Gd-PFH-NAs showed excellent stability in water, phosphate-buffered saline (PBS), and 20% rat serum. Transmission electron microscopy (TEM) images also revealed the effective construction of C-Gd-PFH-NAs as common spherical assemblies. The incubation of C625 thyroid carcinoma with C-Gd-PFH-NAs triggers apoptosis, as confirmed by flow cytometry analysis. The C-Gd-PFH-NAs exhibited antitumor efficacy in human thyroid carcinoma xenografts, where histopathological results further confirmed these outcomes. Furthermore, we were able to use low-intensity focused ultrasound diagnosis imaging (LIFUS) to examine the efficiency of C-Gd-PFH-NAs in thyroid carcinoma . These findings clearly show that the use of LIFUS agents with high performance imaging in different therapeutic settings will have extensive potential for future biomedical applications.
我们报告了包含西妥昔单抗缀合物钆-全氟己烷纳米组装体（C-Gd-PFH-NAs）的纳米组装体的制备，用于甲状腺癌的低强度聚焦超声诊断消融。C-Gd-PFH-NAs在水、磷酸盐缓冲盐水（PBS）和20%大鼠血清中表现出优异的稳定性。透射电子显微镜（TEM）图像也显示C-Gd-PFH-NAs有效构建为常见的球形组装体。流式细胞术分析证实，C625甲状腺癌细胞与C-Gd-PFH-NAs共孵育会引发细胞凋亡。C-Gd-PFH-NAs在人甲状腺癌异种移植瘤中表现出抗肿瘤功效，组织病理学结果进一步证实了这些结果。此外，我们能够使用低强度聚焦超声诊断成像（LIFUS）来检测C-Gd-PFH-NAs在甲状腺癌中的效率。这些发现清楚地表明，在不同治疗环境中使用具有高性能成像的LIFUS剂在未来生物医学应用中将具有广泛的潜力。
