Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853-5904, USA.
Present address: USDA-ARS, Crop Improvement and Protection Research Unit, Salinas, CA. 93905, USA.
J Gen Virol. 2021 Mar;102(3). doi: 10.1099/jgv.0.001576. Epub 2021 Mar 12.
Single aphids can simultaneously or sequentially acquire and transmit multiple potato virus Y (PVY) strains. Multiple PVY strains are often found in the same field and occasionally within the same plant, but little is known about how PVY strains interact in plants or in aphid stylets. Immuno-staining and confocal microscopy were used to examine the spatial and temporal dynamics of PVY strain mixtures (PVY and PVY or PVY and PVY) in epidermal leaf cells of 'Samsun NN' tobacco and 'Goldrush' potato. Virus binding and localization was also examined in aphid stylets following acquisition. Both strains systemically infected tobacco and co-localized in cells of all leaves examined; however, the relative amounts of each virus changed over time. Early in the tobacco infection, when mosaic symptoms were observed, PVY dominated the infection although PVY was detected in some cells. As the infection progressed and vein necrosis developed, PVY was prevalent. Co-localization of PVY and PVY was also observed in epidermal cells of potato leaves with most cells infected with both viruses. Furthermore, two strains could be detected binding to the distal end of aphid stylets following virus acquisition from a plant infected with a strain mixture. These data are in contrast with the traditional belief of spatial separation of two closely related potyviruses and suggest apparent non-antagonistic interaction between PVY strains that could help explain the multitude of emerging recombinant PVY strains discovered in potato in recent years.
单个蚜虫可以同时或顺序获得和传播多种马铃薯 Y 病毒（PVY）株系。在同一田间和偶尔在同一植株内经常发现多种 PVY 株系，但对 PVY 株系在植物内或蚜虫口针内如何相互作用知之甚少。免疫染色和共聚焦显微镜用于研究 'Samsun NN' 烟草和 'Goldrush' 马铃薯表皮叶细胞中 PVY 株系混合物（PVY 和 PVY 或 PVY 和 PVY）的时空动态。在获得后还检查了蚜虫口针中病毒的结合和定位。两种株系均可系统感染烟草，并在所有检查叶片的细胞中共定位；然而，每种病毒的相对数量随时间而变化。在烟草感染的早期，当观察到花叶症状时，PVY 占主导地位，尽管在一些细胞中检测到 PVY。随着感染的进展和叶脉坏死的发展，PVY 流行。在马铃薯叶片的表皮细胞中也观察到 PVY 和 PVY 的共定位，大多数细胞同时感染了两种病毒。此外，在从感染株系混合物的植物中获得病毒后，可以检测到两种株系结合到蚜虫口针的远端。这些数据与两种密切相关的 potyviruses 空间分离的传统观念形成对比，并表明 PVY 株系之间存在明显的非拮抗相互作用，这可以帮助解释近年来在马铃薯中发现的大量新兴重组 PVY 株系。
