棉酚对大鼠睾丸核蛋白的影响。I. 生精细胞中碱性蛋白含量的降低。

The effects of gossypol on nuclear protein in rat testes. I. Reduction in the content of basic proteins in the spermiogenic cells.

作者信息

Fei R R, Teng C S

机构信息

Department of Anatomy, Physiological Science and Radiology, North Carolina State University, Raleigh.

出版信息

Contraception. 1988 Mar;37(3):279-90. doi: 10.1016/0010-7824(88)90030-3.

DOI:10.1016/0010-7824(88)90030-3

PMID:3370999

Abstract

In rat testes, after 45 days of gossypol treatment, the number of round spermatids (RS) reduced from 9.8 x 10(6) to 6.2 x 10(6) per testis. The number of elongating spermatids (ES) reduced from 6.4 x 10(6) to 3.1 x 10(6) per testis. Total nuclear basic proteins (TNBP, i.e., somatic type of histones H1, H2A,B, H3, and H4, plus testis-specific proteins TP1,2,3, and sperm-specific S1 proteins) were extracted from RS and ES. Gossypol has no detectable effect on the concentration of TNBP in RS. However, significant effect was found in ES, where TNBP was reduced from 8.7 micrograms to 6.8 micrograms/10(6) cells. A two-stage polyacrylamide gel has been used to separate the TNBP and the individual proteins were quantified by a Beckman Model DU-7 computer-monitored spectrophotometer. In the RS cells, the content of the TNBP was not sensitive to gossypol. However, in the ES cells, the content of histones H1, H2A, and H2B were reduced by gossypol treatment (with an inhibition of 50%, 58%, and 31%, respectively). The inhibition on H3, TP1, TP3 and S1 was insignificant by gossypol.

摘要

在大鼠睾丸中，棉酚处理45天后，每个睾丸的圆形精子细胞（RS）数量从9.8×10⁶减少至6.2×10⁶。每个睾丸的伸长精子细胞（ES）数量从6.4×10⁶减少至3.1×10⁶。从圆形精子细胞和伸长精子细胞中提取总核碱性蛋白（TNBP，即体细胞类型的组蛋白H1、H2A、B、H3和H4，加上睾丸特异性蛋白TP1、2、3和精子特异性S1蛋白）。棉酚对圆形精子细胞中TNBP的浓度没有可检测到的影响。然而，在伸长精子细胞中发现了显著影响，其中TNBP从8.7微克减少至6.8微克/10⁶个细胞。使用双阶段聚丙烯酰胺凝胶分离TNBP，并通过贝克曼DU-7型计算机监控分光光度计对单个蛋白质进行定量。在圆形精子细胞中，TNBP的含量对棉酚不敏感。然而，在伸长精子细胞中，棉酚处理使组蛋白H1、H2A和H2B的含量降低（抑制率分别为50%、58%和31%）。棉酚对H3、TP1、TP3和S1的抑制作用不显著。