玻璃化法冷冻保存大鼠囊胚

Cryopreservation of rat blastocysts by vitrification.

作者信息

Kono T, Suzuki O, Tsunoda Y

机构信息

National Institute of Animal Industry, Ibaraki, Japan.

出版信息

Cryobiology. 1988 Apr;25(2):170-3. doi: 10.1016/0011-2240(88)90011-9.

DOI:10.1016/0011-2240(88)90011-9

PMID:3371062

Abstract

Rat blastocysts equilibrated with vitrification solution (VS1), consisting of dimethyl sulfoxide, acetamide, propylene glycol, and polyethylene glycol were plunged directly into liquid nitrogen. The embryo suspension are solidified by an extreme elevation in viscosity of solution. The embryos are cryopreserved by vitrification without intra- and extracellular ice formation. The proportion of morphologically normal embryos after cooling and warming was 79% (117/149) and all (48/48) of the embryos cultured were developed to expanded or hatched blastocysts. Normal live young were obtained 41% of the time (28/69) after transfer of the cooled and warmed embryos to pseudopregnant recipients.

摘要

用由二甲亚砜、乙酰胺、丙二醇和聚乙二醇组成的玻璃化溶液（VS1）平衡后的大鼠囊胚直接投入液氮中。胚胎悬液因溶液粘度极度升高而固化。胚胎通过玻璃化冷冻保存，不会形成细胞内和细胞外冰晶。冷却和复温后形态正常的胚胎比例为79%（117/149），所有培养的胚胎（48/48）均发育为扩张或孵化的囊胚。将冷却和复温后的胚胎移植到假孕受体后，41%的时间（28/69）获得了正常的活仔。