Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, China.
J Appl Microbiol. 2021 Oct;131(4):1683-1694. doi: 10.1111/jam.15064. Epub 2021 Apr 24.
The present study is aimed to reveal the variations in epiphytic microbial composition among six whole crop corn (WCC) varieties and their contributions on ensiling characteristics and microbial composition of WCC silage.
Six WCC varieties (JS06, YS23, BS20, JS39, JS40 and JS26) were ensiled for 90 days. All WCC varieties were well fermented with low pH value (<4·0) and high LA (73·6-124 g kg DM, dry matter) concentration. Of six varieties, JS40 had the highest LA (124 g kg DM) concentration, which was supported by highest relative abundance of Lactobacillus. Pantoea was the most dominant epiphytic bacteria in all fresh WCC varieties; however, the secondary dominant genera among six WCC were absolutely difference. Lactobacillus became predominant genus in 90-day silages except YS23. YS23 kept the more bacterial genus from fresh to 90-day silages than other silages, meanwhile Acinetobacter and Enterobacter were the dominant bacteria in YS23 silages.
Among six WCC varieties, JS40 silage had the highest LA. The variations in epiphytic microbiomes among fresh WCC affected terminal microbial community of 90-day silages. There were differences in fermentation characteristics among six WCC varieties, which might be partly attributed to variations in epiphytic microbiomes among fresh WCC.
The study not only enriches the research on microbial communities of plant phyllosphere but also provides theoretical basis for selecting WCC varieties and inoculants for the forage production.
本研究旨在揭示 6 种全株玉米（WCC）品种间附生微生物组成的变化及其对 WCC 青贮特性和微生物组成的贡献。
将 6 种 WCC 品种（JS06、YS23、BS20、JS39、JS40 和 JS26）青贮 90 天。所有 WCC 品种均发酵良好，pH 值（<4.0）低，LA（73.6-124g/kg DM，干物质）浓度高。在这 6 个品种中，JS40 的 LA（124g/kg DM）浓度最高，这得益于其乳酸菌相对丰度最高。在所有新鲜 WCC 品种中，泛菌属是最主要的附生细菌；然而，6 种 WCC 之间的次要优势属绝对不同。除 YS23 外，乳酸菌在 90 天青贮中成为主要属。与其他青贮相比，YS23 在从新鲜到 90 天青贮中保持了更多的细菌属，同时，不动杆菌属和肠杆菌属是 YS23 青贮中的优势细菌。
在 6 种 WCC 品种中，JS40 青贮的 LA 最高。新鲜 WCC 附生微生物组的变化影响 90 天青贮的终末微生物群落。6 种 WCC 品种之间的发酵特性存在差异，这可能部分归因于新鲜 WCC 之间附生微生物组的差异。
该研究不仅丰富了植物叶际微生物群落的研究，也为选择 WCC 品种和接种剂进行饲料生产提供了理论依据。
