Dean N C, Golden J A, Evans L A, Warnock M L, Addison T E, Hopewell P C, Levy J A
Medical Service, San Francisco General Hospital Medical Center.
Chest. 1988 Jun;93(6):1176-9. doi: 10.1378/chest.93.6.1176.
We cultured bronchoalveolar lavage fluid for the human immunodeficiency virus (HIV) from 23 consecutive patients with acquired immunodeficiency syndrome (AIDS) and pulmonary symptoms. We also included a nonconsecutive AIDS patient with recent worsening of respiratory symptoms who had had lymphocytic interstitial pneumonitis (LIP) diagnosed six months earlier. Infectious HIV was present in the cellular fraction from two of the 23 consecutive patients and in the patient with LIP. No virus was isolated from the cell-free portion of the centrifuged fluids. The patients from whom HIV was cultured were not distinguishable from other patients by clinical, radiographic, or laboratory data, and their subsequent course did not appear to differ. One patient with a positive HIV culture had organizing pneumonia without evidence of LIP at autopsy three weeks after lavage. This study demonstrates that HIV can be cultured from cells obtained by bronchoalveolar lavage and suggests that its presence is not associated with a single specific pulmonary histologic pattern.
我们对23例连续的获得性免疫缺陷综合征（AIDS）且有肺部症状的患者的支气管肺泡灌洗（BAL）液进行了人类免疫缺陷病毒（HIV）培养。我们还纳入了1例非连续的AIDS患者，该患者近期呼吸道症状恶化，6个月前被诊断为淋巴细胞间质性肺炎（LIP）。在23例连续患者中的2例以及LIP患者的细胞成分中发现了具有传染性的HIV。从离心后的无细胞部分未分离出病毒。培养出HIV的患者在临床、影像学或实验室数据方面与其他患者并无差异，且他们随后的病程似乎也没有不同。1例HIV培养阳性的患者在灌洗3周后尸检时发现有机化性肺炎，无LIP证据。本研究表明，HIV可从支气管肺泡灌洗获得的细胞中培养出来，并提示其存在与单一特定的肺部组织学模式无关。
