林可霉素对厌氧消化的影响：污泥类型、沼气生成、产甲烷途径和抗性机制。
Influence of lincomycin on anaerobic digestion: Sludge type, biogas generation, methanogenic pathway and resistance mechanism.
机构信息
Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China.
Key Laboratory of Yangtze River Water Environment, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China.
出版信息
Bioresour Technol. 2021 Jun;329:124913. doi: 10.1016/j.biortech.2021.124913. Epub 2021 Feb 28.
This study investigated the tolerance, defensive response and methanogenic pathways of anaerobic granular slugde and anaerobic suspended sludge (AGS and ASS) exposed to different LCM concentrations. AGS presented a higher tolerance to LCM stress, accompanied with 20.8 ± 2.6% enhancement in methane production at 1000 mg/L LCM, which was likely attributed to the less cell deaths and extracellular polymeric substances (EPSs) protection. In the acidification stage, acetate accumulation was stimulated and the activity of acetate kinase was promoted by LCM. In the methanogenesis stage, propionate and butyrate utilization for methane production were impaired after LCM addition. LCM also improved the activity of pyruvate-ferredoxin oxidoreductase and strengthened the process of hydrogenotrophic methanogenesis, likely by accelerating interspecies electron transfer mediated by hydrogen. ErmB and ermF were the dominate LCM resistance genes in AGS under LCM pressure conferring the resistance mechanism of ribosomal protection.
本研究考察了厌氧颗粒污泥和厌氧悬浮污泥（AGS 和 ASS）在不同 LCM 浓度下的耐受性、防御反应和产甲烷途径。AGS 对 LCM 胁迫表现出更高的耐受性，在 1000mg/L LCM 下甲烷产量提高了 20.8±2.6%，这可能归因于细胞死亡较少和细胞外聚合物（EPS）的保护。在酸化阶段，LCM 刺激了乙酸的积累，并促进了乙酸激酶的活性。在产甲烷阶段，添加 LCM 后，丙酸和丁酸用于产甲烷的利用受到损害。LCM 还提高了丙酮酸-铁氧还蛋白氧化还原酶的活性，并加强了氢营养型产甲烷过程，可能通过加速由氢介导的种间电子转移。在 LCM 压力下，AGS 中的 ErmB 和 ermF 是主要的 LCM 抗性基因，赋予了核糖体保护的抗性机制。
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