Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea.
Department of Aquaculture, Sylhet Agricultural University, Sylhet-3100, Bangladesh.
Probiotics Antimicrob Proteins. 2021 Oct;13(5):1277-1291. doi: 10.1007/s12602-021-09749-9. Epub 2021 Mar 13.
Experiments were conducted to identify different ratios of Bacillus sp. SJ-10 and Lactobacillus plantarum KCCM 11322 mixtures at a concentration of 1 × 10 CFU/g diet; the effects on growth and cellular and humoral immune responses and the characteristics of disease protection in olive flounder (Paralichthys olivaceus). Flounder were divided into six groups and fed control diet D-1 (without Bacillus sp. SJ-10 and L. plantarum KCCM 11322), positive control diets D-2 (Bacillus sp. SJ-10 at 1 × 10 CFU/g feed) and D-3 (L. plantarum KCCM 11322 at 1 × 10 CFU/g feed); or treatment diets D-4 (3:1 Bacillus sp. SJ-10 and L. plantarum KCCM 11322 at 0.75 + 0.25 × 10 CFU/g feed), D-5 (1:1 Bacillus sp. SJ-10 and L. plantarum KCCM 11322 at 0.50 + 0.50 × 10 CFU/g feed), or D-6 (1:3 Bacillus sp. SJ-10 and L. plantarum KCCM 11322 at 0.25 + 0.75 × 10 CFU/g feed) for 8 weeks. Group D-4 demonstrated better growth and feed utilization (P < 0.05) compared with the controls and positive controls. Similar modulation was also observed in respiratory burst for all treatments and in the expression levels of TNF-α, IL-1β, IL-6, and IL-10 in different organs in D-4. D-4 and D-5 increased respiratory burst, superoxide dismutase, lysozyme, and myeloperoxidase activities compared with the controls, and only D-4 increased microvilli length. When challenged with 1 × 10 CFU/mL Streptococcus iniae, the fish in the D-4 and D-5 groups survived up to 14 days, whereas the fish in the other groups reached 100% mortality at 11.50 days. Collectively, a ratio-specific Bacillus sp. SJ-10 and L. plantarum KCCM 11322 mixture (3:1) was associated with elevated growth, innate immunity, and streptococcosis resistance (3:1 and 1:1) compared with the control and single probiotic diets.
实验旨在确定不同比例的枯草芽孢杆菌 SJ-10 和植物乳杆菌 KCCM 11322 混合物在饲料中浓度为 1×10 CFU/g 时，对橄榄石斑鱼（Paralichthys olivaceus）生长、细胞和体液免疫反应以及疾病保护特性的影响。石斑鱼被分为六组，分别投喂对照饲料 D-1（不含枯草芽孢杆菌 SJ-10 和植物乳杆菌 KCCM 11322）、阳性对照饲料 D-2（饲料中枯草芽孢杆菌 SJ-10 的浓度为 1×10 CFU/g）和 D-3（饲料中植物乳杆菌 KCCM 11322 的浓度为 1×10 CFU/g）；或处理饲料 D-4（枯草芽孢杆菌 SJ-10 和植物乳杆菌 KCCM 11322 的比例为 3:1，浓度为 0.75+0.25×10 CFU/g 饲料）、D-5（枯草芽孢杆菌 SJ-10 和植物乳杆菌 KCCM 11322 的比例为 1:1，浓度为 0.50+0.50×10 CFU/g 饲料）或 D-6（枯草芽孢杆菌 SJ-10 和植物乳杆菌 KCCM 11322 的比例为 1:3，浓度为 0.25+0.75×10 CFU/g 饲料），为期 8 周。与对照组和阳性对照组相比，D-4 组表现出更好的生长和饲料利用率（P<0.05）。在所有处理组中，D-4 组还观察到呼吸爆发和 TNF-α、IL-1β、IL-6 和 IL-10 在不同器官中的表达水平也得到了类似的调节。与对照组相比，D-4 和 D-5 组的呼吸爆发、超氧化物歧化酶、溶菌酶和髓过氧化物酶活性增加，只有 D-4 组的微绒毛长度增加。当用 1×10 CFU/mL 鳗弧菌攻毒时，D-4 和 D-5 组的鱼存活时间长达 14 天，而其他组的鱼在 11.50 天达到 100%死亡率。总的来说，与对照组和单一益生菌饲料相比，枯草芽孢杆菌 SJ-10 和植物乳杆菌 KCCM 11322 的特定比例混合物（3:1）与生长、先天免疫和链球菌病抗性的提高有关（3:1 和 1:1）。
