Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Dongzhimennei, Beijing, China.
Wang Jing Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
Biosci Biotechnol Biochem. 2021 May 25;85(6):1332-1340. doi: 10.1093/bbb/zbab038.
Owing to the high degree of diversity of metabolite pools and complexity of spatial and temporal distributions within biological tissues, currently available methods for metabolite characterization face large challenges. In this study, the temporal and spatial distributions of the alkaloid components of the medicinal plant lotus (Nelumbo nucifera) were investigated over various growth phases. The results showed that alkaloid biosynthesis in lotus leaf is regulated by development and that there is maximum accumulation of alkaloids when the lotus leaf was completely expanded. Furthermore, alkaloid content tended to be stable in mature lotus leaves. However, there was significant variation in the alkaloid content of lotus leaves with different genotypes, suggesting that genetic background is an important factor that affects the temporal and spatial distributions of alkaloids in sacred lotus leaves. The dynamic contents of alkaloids during the growth and development of lotus leaves provide insight into basic biological differences when sampling.
由于代谢物库的高度多样性以及生物组织内时空分布的复杂性，目前用于代谢物特征描述的方法面临着巨大的挑战。在这项研究中，对药用植物荷叶（Nelumbo nucifera）的生物碱成分在不同生长阶段的时空分布进行了研究。结果表明，荷叶中的生物碱生物合成受发育调控，当荷叶完全展开时，生物碱积累最多。此外，成熟荷叶中的生物碱含量趋于稳定。然而，不同基因型荷叶的生物碱含量存在显著差异，这表明遗传背景是影响荷叶中生物碱时空分布的重要因素。荷叶生长发育过程中生物碱含量的动态变化为采样时提供了基本的生物学差异的见解。
