V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
V. I. Kulakov National Medical Research Centre of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2021 Feb;170(4):515-521. doi: 10.1007/s10517-021-05099-2. Epub 2021 Mar 13.
Almost quarter of a century long studies aimed at identification, isolation, culturing, and use of postnatal pluripotent cells for the development of cell-based technologies have not met with success and failed to provide reliable and reproducible protocols of cell isolation, identification, and culturing. At the same time, experimental data in this field suggest that postnatal pluripotent cells are not the copies of embryonic cells and, therefore, the tests routinely used for identification of embryonic pluripotent cells are not fully adequate for characterization of their postnatal analogues. Therefore, cell lineage tracing methods showing the differentiation routes of the studied cells in human or animal body after birth should be developed and used.
长达四分之一世纪的研究旨在鉴定、分离、培养和利用多能干细胞，以开发基于细胞的技术，但这些研究都没有成功，也未能提供可靠和可重复的细胞分离、鉴定和培养方案。与此同时，该领域的实验数据表明，多能干细胞不是胚胎细胞的复制品，因此，常规用于鉴定胚胎多能细胞的测试方法并不完全适用于其出生后类似物的特征描述。因此，应该开发和使用细胞谱系示踪方法来显示研究细胞在人体或动物体内出生后的分化途径。
