Group of Fish Health and Infectious Diseases, Universitary Institute of Animal Health and Food Safety (IUSA), University of Las Palmas de Gran Canaria, Carretera de Trasmontaña s/n, 35416, Arucas, Spain.
CIBER de Enfermedades Respiratorias, Instituto de Salud Carlos III, Monforte de Lemos 3-5, Pabellón 11, 28029, Madrid, Spain.
Probiotics Antimicrob Proteins. 2021 Oct;13(5):1326-1337. doi: 10.1007/s12602-021-09767-7. Epub 2021 Mar 13.
A strain of Alcaligenes faecalis A12C (A. faecalis A12C) isolated from Argyrosomus regius is a probiotic in fish. Previous experiments showed that A. faecalis A12C had inhibitory effects on the growth of multidrug-resistant bacteria. We aimed to confirm whether A. faecalis A12C is safe and has adequate intestinal colonization in experimental rats, and evaluate its efficacy in an animal model of peritonitis. We used 30 male rats, randomly divided into 6 groups (n = 5): three groups (HA7, HA15, HA30) received A. faecalis A12C in drinking water (6 × 10 CFU/mL) for 7 days, and three control groups received drinking water only. All groups were evaluated at 7, 15, and 30 days. Survival after A. faecalis A12C administration was 100% in all groups. Mild eosinophilia (1.5%, p < 0.01) and increased aspartate aminotransferase (86 IU/L, p < 0.05) were observed in HA7, followed by progressive normalization. No histological signs of organ injury were found. We observed significant E. coli decline in faeces, parallel to an increase in A. faecalis A12C at 7 days. E. coli had a tendency to recover initial values, while A. faecalis A12C disappeared from the intestinal microbiota at 30 days. To evaluate its efficacy against peritonitis, we studied two additional groups of animals: IA group pretreated with A. faecalis A12C before E. coli intra-abdominal inoculation, and IC group inoculated with no A. faecalis A12C. We found an increase in C-reactive protein, alanine aminotransferase, urea, and eosinophils in IC animals when compared with IA. Peritonitis was more evident in IC than in IA animals. Our findings suggest that A. faecalis A12C altered clinically relevant parameters in sepsis and was associated with a lesser spread of infection.
一株从黄姑鱼中分离得到的产碱杆菌 A12C（A. faecalis A12C）是鱼类中的一种益生菌。先前的实验表明，A. faecalis A12C 对多重耐药菌的生长具有抑制作用。我们旨在确认 A. faecalis A12C 在实验大鼠中是否安全且具有足够的肠道定植能力，并评估其在腹膜炎动物模型中的疗效。我们使用 30 只雄性大鼠，随机分为 6 组（n=5）：3 组（HA7、HA15、HA30）连续 7 天通过饮用水摄入 A. faecalis A12C（6×10 CFU/mL），3 组仅接受饮用水。所有组均在第 7、15 和 30 天进行评估。所有组大鼠在接受 A. faecalis A12C 治疗后均存活 100%。在 HA7 中观察到轻度嗜酸性粒细胞增多（1.5%，p<0.01）和天冬氨酸氨基转移酶升高（86 IU/L，p<0.05），随后逐渐恢复正常。未发现器官损伤的组织学迹象。我们观察到粪便中大肠杆菌显著减少，同时在第 7 天 A. faecalis A12C 增加。大肠杆菌有恢复初始值的趋势，而 A. faecalis A12C 在第 30 天从肠道菌群中消失。为了评估其对腹膜炎的疗效，我们研究了另外两组动物：IA 组在大肠杆菌腹腔内接种前用 A. faecalis A12C 预处理，IC 组不接种 A. faecalis A12C。我们发现与 IA 组相比，IC 组动物的 C 反应蛋白、丙氨酸氨基转移酶、尿素和嗜酸性粒细胞增加。IC 组动物的腹膜炎比 IA 组更明显。我们的研究结果表明，A. faecalis A12C 改变了败血症中临床相关参数，并与感染的传播减少有关。
