Department of Basic Medicine and Forensic Medicine, Hangzhou Medical College, Hangzhou, P.R. China.
Department of Basic Medicine, Medical school of ZheJiang University, Hangzhou, P.R. China.
Int J Dev Neurosci. 2021 Jun;81(4):312-323. doi: 10.1002/jdn.10102. Epub 2021 Mar 18.
In order to understand the role of long noncoding RNAs (lncRNAs) played in the mechanisms of glyphosate neurotoxicity in neuronal development.
Perinatal glyphosate exposure (PGE) mouse model was constructed, and a lncRNA microarray was used to study the lncRNA expression changes in the hippocampus tissue of perinatal glyphosate exposure mice. Then we used GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) databases to analyze the function of the differentially expressed mRNAs and lncRNAs.
LncRNA microarray analysis revealed that 1759 lncRNAs and 759 mRNAs were differentially expressed in the perinatal glyphosate exposure (PGE) mice group (G group) compared with the normal control mice group (C group). The functions of the DEmRNAs are involved in the cellular response to hormone stimulus. The ceRNA analysis showed that some interaction networks existed, including (ENSMUST00000137546, ENSMUST00000160950)/(miR-34a-3p, miR-130a-3p)/(Il12b, Irf1). Further analysis of the target mRNAs of miRNAs indicated that the possible functions involved the neuroactive ligand-receptor interaction and calcium signaling pathway, which are involved in perinatal glyphosate exposure-induced neurotoxicity.
The aberrant expression of lncRNAs is related to the perinatal glyphosate-exposed neurotoxicity. These lncRNAs affect the target gene expression level, might by regulating neuroactive ligand-receptor interactions. The (ENSMUST00000137546, ENSMUST00000160950)/ (miRNA-34a-5p, miR-130a-3p) / mRNAs (e.g., Il12b, Irf1) interaction network may functions in perinatal glyphosate exposure-induced neurotoxicity.
为了了解长链非编码 RNA（lncRNA）在神经发育过程中草甘膦神经毒性机制中的作用。
构建围产期草甘膦暴露（PGE）小鼠模型，使用 lncRNA 微阵列研究围产期草甘膦暴露小鼠海马组织中的 lncRNA 表达变化。然后，我们使用 GO（基因本体论）和 KEGG（京都基因与基因组百科全书）数据库来分析差异表达的 mRNAs 和 lncRNAs 的功能。
lncRNA 微阵列分析显示，与正常对照组（C 组）相比，围产期草甘膦暴露（PGE）组（G 组）的 1759 个 lncRNAs 和 759 个 mRNAs 表达存在差异。差异表达的 mRNAs 的功能涉及细胞对激素刺激的反应。ceRNA 分析表明存在一些相互作用网络，包括（ENSMUST00000137546，ENSMUST00000160950）/（miR-34a-3p，miR-130a-3p）/（Il12b，Irf1）。进一步分析 miRNA 的靶 mRNAs 表明，可能涉及的功能包括神经活性配体-受体相互作用和钙信号通路，这些通路涉及围产期草甘膦暴露引起的神经毒性。
lncRNAs 的异常表达与围产期草甘膦暴露引起的神经毒性有关。这些 lncRNAs 影响靶基因的表达水平，可能通过调节神经活性配体-受体相互作用。（ENSMUST00000137546，ENSMUST00000160950）/（miR-34a-3p，miR-130a-3p）/mRNAs（如 Il12b，Irf1）的相互作用网络可能在围产期草甘膦暴露引起的神经毒性中发挥作用。
