Department of Physiology, Laboratory for Reproductive Biology and Developmental Programming, Edo State University Uzairue, Iyamho, Edo State, Nigeria.
Department of Anatomy, Faculty of Biomedical Sciences, Kampala International University, Western Campus, Bushenyi, Uganda.
J Basic Clin Physiol Pharmacol. 2021 Mar 15;33(3):297-303. doi: 10.1515/jbcpp-2020-0281.
Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that . oil has a wide range of biological effects, this study aimed at investigating the effect of oil on lead acetate-induced reproductive toxicity in male Wistar rats.
Twenty (20) sexually matured male Wistar rats (55-65 days) were randomly distributed into four groups (n=5). Group I (negative control)-distilled water orally for 56 days, Group II (positive control)-5 mg/kg bwt lead acetate intraperitoneally for 14 days, Group III-6.7 mL/kg bwt . oil orally for 56 days and Group IV-lead acetate intraperitoneally for 14 days and . oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis.
The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group.
oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.
铅主要通过激素失衡和睾丸组织形态损伤对男性生殖功能产生影响，导致精子形态和氧化标志物发生显著变化。然而，不同的研究表明，[油的名称]具有广泛的生物学效应，本研究旨在探讨[油的名称]对醋酸铅诱导的雄性 Wistar 大鼠生殖毒性的影响。
将 20 只（55-65 天）性成熟雄性 Wistar 大鼠随机分为四组（n=5）。第 I 组（阴性对照组）-口服蒸馏水 56 天，第 II 组（阳性对照组）-腹腔内注射 5mg/kg bwt 醋酸铅 14 天，第 III 组-口服 6.7mL/kg bwt [油的名称]56 天，第 IV 组-腹腔内注射醋酸铅 14 天，口服 [油的名称]56 天。大鼠用二乙醚处死，收集血清、睾丸和附睾进行精液分析、生化和组织学分析。
醋酸铅显著增加（p<0.05）睾丸和附睾丙二醛（MDA）水平，与阴性对照组相比，精子参数、器官重量、睾酮和促黄体生成素显著降低（p<0.05）。与阳性对照组相比，[油的名称]与醋酸铅共同给药显著增加（p<0.05）睾酮、促黄体生成素、精子参数和器官重量，MDA 水平显著降低（p<0.05）。组织学分析显示，醋酸铅破坏睾丸细胞结构和生精细胞完整性，而在共同处理组中则得到了正常化。
[油的名称]减轻了醋酸铅对雄性 Wistar 大鼠的有害影响，这可能归因于其多酚含量和抗氧化特性。
