非常见原因：多聚免疫球蛋白 A 副蛋白导致溶液中微粒（KIMS）免疫分析的动力学相互作用出现假阳性结果。

Not the usual suspect: Polymeric IgA paraprotein causes false positive results in kinetic interaction of microparticles in solution (KIMS) immunoassays.

机构信息

Department of Pathology and Laboratory Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, United States.

出版信息

Clin Biochem. 2021 Jun;92:82-85. doi: 10.1016/j.clinbiochem.2021.03.001. Epub 2021 Mar 11.

DOI:10.1016/j.clinbiochem.2021.03.001

PMID:33713635

Abstract

Immunoassays are commonly used by the clinical laboratory, but paraproteins can occasionally produce erroneous results. In this study, we investigated the cause of apparent false positive results for multiple Kinetic Interaction of Microparticles in Solution (KIMS) immunoassays. Patient controls and samples containing the interference were analyzed using automated chemistry platforms, gel electrophoresis, immunofixation, affinity chromatography, and size exclusion chromatography. Our results show that IgA paraprotein caused false positive results for the KIMS measurement of three therapeutic drugs. To our knowledge, this is the first report of IgA paraprotein-causing immunoassay interference. The clinical implications of this interference are discussed.

摘要

免疫测定法在临床实验室中被广泛应用，但某些情况下，副蛋白可能会导致错误的结果。在本研究中，我们调查了造成多个 Kinetic Interaction of Microparticles in Solution (KIMS) 免疫测定法出现明显假阳性结果的原因。我们使用自动化化学平台、凝胶电泳、免疫固定、亲和层析和分子筛层析对患者对照和含有干扰的样本进行了分析。结果表明，IgA 副蛋白导致 KIMS 对三种治疗药物的测量出现假阳性结果。据我们所知，这是首次报道 IgA 副蛋白引起免疫测定干扰的情况。我们还讨论了这种干扰的临床意义。