Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, 210023, PR China.
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, PR China.
Biosens Bioelectron. 2021 Jun 1;181:113149. doi: 10.1016/j.bios.2021.113149. Epub 2021 Mar 5.
Chlorothalonil (CHL), imidacloprid (IMI) and oxyfluorfen (OXY) are commonly used in combination to increase crop yield. However, these three pesticides are toxic to aquatic organisms and do not easily degrade. In this study, a surface-enhanced Raman scattering-based lateral flow assay (SERS-LFA) test strip was prepared by combining antibodies with SERS nanotags, and then competitive immune binding was used to detect the three pesticides simultaneously. Moreover, the two-way binding effect of ssDNA-streptavidin bound to Ag@AuNPs and Ag@AuNPs with antibodies was used to further amplify the detection signal. Under the optimal conditions, the SERS-LFA test strips exhibited high sensitivity, a low detection limit, short detection time, high specificity and low cost. Furthermore, the detection range was within the values prescribed by international detection standards. By measuring the intensity of the SERS signal on the test line of the paper strip, accurate quantitative analysis was achieved. The practical application of the proposed system was demonstrated by simultaneous detection of CHL, IMI and OXY in environmental and food samples with satisfactory results.
百菌清（CHL）、氯吡虫啉（IMI）和三氟羧草醚（OXY）常被联合使用以提高作物产量。然而，这三种农药对水生生物具有毒性且不易降解。在本研究中，通过将抗体与 SERS 纳米标签结合，制备了基于表面增强拉曼散射的侧向流动检测（SERS-LFA）试纸条，然后通过竞争免疫结合来同时检测这三种农药。此外，通过将 ssDNA-链霉亲和素结合到 Ag@AuNPs 和带有抗体的 Ag@AuNPs 上来进一步放大检测信号，利用了双结合效应。在最佳条件下，SERS-LFA 试纸条表现出高灵敏度、低检测限、短检测时间、高特异性和低成本。此外，检测范围在国际检测标准规定的范围内。通过测量纸条检测线上的 SERS 信号强度，实现了准确的定量分析。通过对环境和食品样品中 CHL、IMI 和 OXY 的同时检测，证明了所提出系统的实际应用，结果令人满意。
