MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Integrative Biology of Marine Models (LBI2M, UMR8227), Station Biologique deRoscoff (SBR), CNRS, Sorbonne Université, 29680, Roscoff, France.
J Phycol. 2021 Aug;57(4):1295-1308. doi: 10.1111/jpy.13167. Epub 2021 Jun 2.
Ulva compressa, a green tide-forming species, can adapt to hypo-salinity conditions, such as estuaries and brackish lakes. To understand the underlying molecular mechanisms of hypo-salinity stress tolerance, transcriptome-wide gene expression profiles in U. compressa were created using digital gene expression profiles. The RNA-seq data were analyzed based on the comparison of differently expressed genes involved in specific pathways under hypo-salinity and recovery conditions. The up-regulation of genes in photosynthesis and glycolysis pathways may contribute to the recovery of photosynthesis and energy metabolism, which could provide sufficient energy for the tolerance under long-term hyposaline stress. Multiple strategies, such as ion transportation and osmolytes metabolism, were performed to maintain the osmotic homeostasis. Additionally, several long noncoding RNA were differently expressed during the stress, which could play important roles in the osmotolerance. Our work will serve as an essential foundation for the understanding of the tolerance mechanism of U. compressa under the fluctuating salinity conditions.
石莼（Ulva compressa）是一种能够适应低盐环境的绿潮形成物种，如河口和半咸水湖泊。为了了解低盐胁迫耐受性的潜在分子机制，使用数字基因表达谱创建了石莼的转录组范围的基因表达谱。根据在低盐和恢复条件下参与特定途径的差异表达基因的比较，对 RNA-seq 数据进行了分析。光合作用和糖酵解途径中基因的上调可能有助于光合作用和能量代谢的恢复，这可为长期低盐胁迫下的耐受性提供足够的能量。通过多种策略，如离子转运和渗透物代谢，来维持渗透平衡。此外，在胁迫过程中还表达了几种不同的长非编码 RNA，它们可能在耐渗性中发挥重要作用。我们的工作将为理解石莼在波动盐度条件下的耐受性机制提供重要基础。
