Zhang Ling, Zhang Shenglan, Wang Jianbin, Li Xiaojing
Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei 056002, P.R. China.
Medical Department, Handan Central Hospital, Handan, Hebei 056002, P.R. China.
Exp Ther Med. 2021 Apr;21(4):296. doi: 10.3892/etm.2021.9727. Epub 2021 Jan 28.
The present study aimed to investigate the expression of microRNA (miR)-146b in psoriatic tissue and its correlation with psoriasis activity and inflammation. The effect of miR-146b overexpression on keratinocyte proliferation and apoptosis was also explored. The expression of miR-146b in the psoriasis-affected tissue and non-affected tissue of 110 patients was determined via reverse transcription-quantitative (RT-q)PCR. The psoriasis-affected body surface area and psoriasis area severity index (PASI) score were recorded for evaluating disease activity. The expression of various inflammatory cytokines in psoriasis-affected tissue was also detected via RT-qPCR. miR-146b overexpression and control plasmids were constructed and transfected into HaCaT cells . Subsequently, cell proliferation, apoptosis and tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-induced cell apoptosis were determined. The results revealed that the expression of miR-146b was increased in psoriasis-affected tissue compared with that in unaffected tissue. The results obtained from a receiver operating characteristic curve analysis demonstrated that miR-146b levels were able to discriminate between psoriasis-affected tissue and unaffected tissue, with an area under the curve value of 0.781 (95% CI: 0.720-0.843). In addition, miR-146b expression in psoriatic tissue was correlated with an increased PASI score in patients with psoriasis. miR-146b expression in psoriatic tissue was positively correlated with TNF-α, interleukin (IL)-6 and IL-17 mRNA levels. , miR-146b overexpression enhanced HaCaT cell proliferation and suppressed apoptosis as well as TRAIL-induced apoptosis when compared with that in control-transfected HaCaT cells. In conclusion, miR-146b was positively correlated with disease activity and psoriatic tissue inflammation. Keratinocyte proliferation was also promoted in psoriasis.
本研究旨在探讨微小RNA（miR）-146b在银屑病组织中的表达及其与银屑病病情活动度和炎症的相关性。同时还探究了miR-146b过表达对角质形成细胞增殖和凋亡的影响。通过逆转录定量（RT-q）PCR测定了110例患者银屑病受累组织和未受累组织中miR-146b的表达。记录银屑病受累体表面积和银屑病面积严重程度指数（PASI）评分以评估疾病活动度。还通过RT-qPCR检测了银屑病受累组织中多种炎性细胞因子的表达。构建了miR-146b过表达质粒和对照质粒并转染至HaCaT细胞。随后，测定细胞增殖、凋亡以及肿瘤坏死因子（TNF）相关凋亡诱导配体（TRAIL）诱导的细胞凋亡。结果显示，与未受累组织相比，miR-146b在银屑病受累组织中的表达增加。受试者工作特征曲线分析结果表明，miR-146b水平能够区分银屑病受累组织和未受累组织，曲线下面积值为0.781（95%CI：0.720-0.843）。此外，银屑病组织中miR-146b的表达与银屑病患者PASI评分升高相关。银屑病组织中miR-146b的表达与TNF-α、白细胞介素（IL）-6和IL-17 mRNA水平呈正相关。与对照转染的HaCaT细胞相比，miR-146b过表达增强了HaCaT细胞增殖，抑制了凋亡以及TRAIL诱导的凋亡。总之，miR-146b与疾病活动度和银屑病组织炎症呈正相关。银屑病中角质形成细胞增殖也得到促进。
