Ci Zheng, Zhang Ying, Wang Yahui, Wu Gaoyang, Hou Mengjie, Zhang Peiling, Jia Litao, Bai Baoshuai, Cao Yilin, Liu Yu, Zhou Guangdong
Research Institute of Plastic Surgery, Wei Fang Medical College, Wei Fang, China.
Shanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Front Cell Dev Biol. 2021 Feb 26;9:638115. doi: 10.3389/fcell.2021.638115. eCollection 2021.
Scaffold-free cartilage-sheet technology can stably regenerate high-quality cartilage tissue . However, uncontrolled shape maintenance and mechanical strength greatly hinder its clinical translation. Decalcified bone matrix (DBM) has high porosity, a suitable pore structure, and good biocompatibility, as well as controlled shape and mechanical strength. In this study, cartilage sheet was prepared into engineered cartilage gel (ECG) and combined with DBM to explore the feasibility of regenerating 3D cartilage with controlled shape and mechanical strength. The results indicated that ECG cultured for 3 days (3 d) and 15 days (15 d) showed good biocompatibility with DBM, and the ECG-DBM constructs successfully regenerated viable 3D cartilage with typical mature cartilage features in both nude mice and autologous goats. Additionally, the regenerated cartilage had comparable mechanical properties to native cartilage and maintained its original shape. To further determine the optimal seeding parameters for ECG, the 3 d ECG regenerated using human chondrocytes was diluted in different concentrations (1:3, 1:2, and 1:1) for seeding and implantation. The results showed that the regenerated cartilage in the 1:2 group exhibited better shape maintenance and homogeneity than the other groups. The current study established a novel mode of 3D cartilage regeneration based on the design concept of steel (DBM)-reinforced concrete (ECG) and successfully regenerated homogenous and mature 3D cartilage with controlled shape and mechanical strength, which hopefully provides an ideal cartilage graft for the repair of various cartilage defects.
无支架软骨片技术能够稳定地再生高质量软骨组织。然而，形状维持不受控制和机械强度问题极大地阻碍了其临床应用转化。脱钙骨基质（DBM）具有高孔隙率、合适的孔隙结构、良好的生物相容性，以及可控制的形状和机械强度。在本研究中，将软骨片制备成工程化软骨凝胶（ECG）并与DBM相结合，以探索再生具有可控形状和机械强度的三维软骨的可行性。结果表明，培养3天（3 d）和15天（15 d）的ECG与DBM表现出良好的生物相容性，并且ECG-DBM构建体在裸鼠和自体山羊体内均成功再生出具有典型成熟软骨特征的有活力的三维软骨。此外，再生软骨具有与天然软骨相当的力学性能，并保持其原始形状。为了进一步确定ECG的最佳接种参数，将用人软骨细胞再生的3 d ECG以不同浓度（1:3、1:2和1:1）稀释后进行接种和植入。结果表明，1:2组再生软骨的形状维持和同质性优于其他组。本研究基于钢（DBM）增强混凝土（ECG）的设计理念建立了一种新型的三维软骨再生模式，并成功再生出形状和机械强度可控的均匀成熟三维软骨，有望为各种软骨缺损的修复提供理想的软骨移植物。
