Institutes of Health Central Plain, The Third Affiliated Hospital of Xinxiang Medical University, Clinical Medical Center of Tissue Engineering and Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai Key Laboratory of Tissue Engineering, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, PR China.
ACS Biomater Sci Eng. 2023 Aug 14;9(8):4994-5005. doi: 10.1021/acsbiomaterials.3c00488. Epub 2023 Jul 26.
Human decalcified bone matrix (HDBM) is a framework with a porous structure and good biocompatibility. Nevertheless, its oversized pores lead to massive cell loss when seeding chondrocytes directly over it. Gelatin (GT) is a type of protein obtained by partial hydrolysis of collagen. The GT scaffold can be prepared from the GT solution through freeze-drying. More importantly, the pore size of the GT scaffold can be controlled by optimizing the concentration of the GT solution. Similarly, when different concentrations of gelatin are combined with HDBM and then freeze-dried, the pore size of the HDBM can be modified to different degrees. In this study, the HDBM framework was modified with 0.3, 0.6, and 0.9%GT, resulting in an improved pore size and adhesion rate. Results showed that the HDBM framework with 0.6%GT (HDBM-0.6%GT) had an average pore size of 200 μm, which was more suitable for chondrocyte seeding. Additionally, our study validated that porcine decalcified bone matrix (PDBM) had a proper pore structure. Chondrocytes were in vitro seeded on the three frameworks for 4 weeks and then implanted in nude mice and autologous goats, respectively. The in vivo cartilage regeneration results showed that HDBM-0.6%GT and PDBM frameworks compensated for the oversized pores of the HDBM framework. Moreover, they showed successfully regenerated more mature cartilage tissue with a certain shape in animals.
人脱钙骨基质（HDBM）是一种具有多孔结构和良好生物相容性的支架。然而，其过大的孔径在直接接种软骨细胞时会导致大量细胞丢失。明胶（GT）是胶原蛋白经部分水解得到的一种蛋白质。GT 支架可通过 GT 溶液的冷冻干燥来制备。更重要的是，通过优化 GT 溶液的浓度，可以控制 GT 支架的孔径。同样，当不同浓度的明胶与 HDBM 结合并冷冻干燥时，HDBM 的孔径可以在不同程度上进行修饰。在本研究中，HDBM 支架用 0.3%、0.6%和 0.9%GT 进行修饰，从而改善了孔径和黏附率。结果表明，含有 0.6%GT 的 HDBM 支架（HDBM-0.6%GT）的平均孔径为 200μm，更适合软骨细胞接种。此外，我们的研究验证了猪脱钙骨基质（PDBM）具有适当的孔径结构。将软骨细胞在体外接种到这三种支架上 4 周，然后分别植入裸鼠和自体山羊体内。体内软骨再生结果表明，HDBM-0.6%GT 和 PDBM 支架弥补了 HDBM 支架过大的孔径。此外，它们在动物体内成功地再生了具有一定形状的更成熟的软骨组织。
