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芘标记载脂蛋白A-I的数据集、模型开发及拟合以监测其无脂形式的寡聚体种类。

Dataset on pyrene-labelled apolipoprotein A-I, model development and fitting to monitor oligomeric species of its lipid-free form.

作者信息

Tárraga Wilson A, Garda Horacio A, Falomir Lockhart Lisandro J, Gonzalez Marina C

机构信息

Instituto de Investigaciones Bioquímicas de La Plata "Rodolfo Brenner" (INIBIOLP), Centro Científico Tecnológico-La Plata, Calle 60 y 120 (1900), La Plata, Argentina.

Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.

出版信息

Data Brief. 2021 Feb 13;35:106829. doi: 10.1016/j.dib.2021.106829. eCollection 2021 Apr.

DOI:10.1016/j.dib.2021.106829
PMID:33718538
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7921477/
Abstract

This article contains data for the self-association of pyrene-labelled single Cys-mutants of apolipoprotein A-I (apoA-I). Mathematical models were developed to characterise the self-association events at different cysteine positions on apoA-I obtained as a function of protein concentration based on the multi-parametric spectrum of pyrene, particularly -value and excimer emissions. The present work complements data related to the article entitled "Analysis of pyrene-labelled apolipoprotein A-I oligomerisation in solution: Spectra deconvolution and changes in -value and excimer formation" Tárraga et al. [1].

摘要

本文包含载脂蛋白A-I（apoA-I）的芘标记单半胱氨酸突变体自缔合的数据。基于芘的多参数光谱，特别是振动带强度值和准分子发射，建立了数学模型，以表征apoA-I上不同半胱氨酸位置的自缔合事件，这些事件是作为蛋白质浓度的函数获得的。本研究补充了与题为《溶液中芘标记的载脂蛋白A-I寡聚化分析：光谱去卷积以及振动带强度值和准分子形成的变化》的文章相关的数据，作者为塔拉加等人[1]。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/cbe391002309/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/c6a0696081a4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/219ae04398b9/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/c8444b00a9bb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/375b58cd6501/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/c3261f2f22c6/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/3c1afd54ecf0/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/1dd54eb0cba6/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/cbe391002309/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/c6a0696081a4/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/219ae04398b9/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/c8444b00a9bb/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/375b58cd6501/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/c3261f2f22c6/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/3c1afd54ecf0/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/1dd54eb0cba6/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5ee/7921477/cbe391002309/gr8.jpg

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Dataset on pyrene-labelled apolipoprotein A-I, model development and fitting to monitor oligomeric species of its lipid-free form.芘标记载脂蛋白A-I的数据集、模型开发及拟合以监测其无脂形式的寡聚体种类。
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本文引用的文献

1
Analysis of pyrene-labelled apolipoprotein A-I oligomerization in solution: Spectra deconvolution and changes in P-value and excimer formation.分析溶液中芘标记载脂蛋白 A-I 寡聚体：光谱解卷积以及 P 值和激基缔合物形成的变化。
Arch Biochem Biophys. 2021 Mar 15;699:108748. doi: 10.1016/j.abb.2020.108748. Epub 2021 Jan 12.
2
Structural insights into amyloid oligomers of the Parkinson disease-related protein α-synuclein.帕金森病相关蛋白α-突触核蛋白淀粉样寡聚体的结构见解
J Biol Chem. 2014 Sep 26;289(39):26733-26742. doi: 10.1074/jbc.M114.566695. Epub 2014 Aug 20.
3
Characterization of a human apolipoprotein a-I construct expressed in a bacterial system.
在细菌系统中表达的人载脂蛋白 A-I 构建体的表征。
Protein J. 2012 Dec;31(8):681-8. doi: 10.1007/s10930-012-9448-z.
4
Pyrene: a probe to study protein conformation and conformational changes.芘：一种研究蛋白质构象和构象变化的探针。
Molecules. 2011 Sep 14;16(9):7909-35. doi: 10.3390/molecules16097909.
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Optimized bacterial expression of human apolipoprotein A-I.人载脂蛋白A-I的优化细菌表达
Protein Expr Purif. 2003 Jan;27(1):98-103. doi: 10.1016/s1046-5928(02)00568-5.
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Decomposition of protein tryptophan fluorescence spectra into log-normal components. I. Decomposition algorithms.将蛋白质色氨酸荧光光谱分解为对数正态分量。I. 分解算法。
Biophys J. 2001 Sep;81(3):1699-709. doi: 10.1016/S0006-3495(01)75823-7.
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A re-examination of the line shape of the electronic spectra of complex molecules in solution: log-normal function versus gaussian.溶液中复杂分子电子光谱线形的重新审视：对数正态函数与高斯函数
Spectrochim Acta A Mol Biomol Spectrosc. 2000 Oct;56A(11):2207-10. doi: 10.1016/s1386-1425(00)00274-2.
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Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants.通过线性外推法测定的去折叠自由能变化。1. 使用不同变性剂时苯甲磺酰α-胰凝乳蛋白酶的去折叠。
Biochemistry. 1988 Oct 18;27(21):8063-8. doi: 10.1021/bi00421a014.
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N-(1-pyrene)maleimide: a fluorescent cross-linking reagent.N-（1-芘基）马来酰亚胺：一种荧光交联试剂。
Biochemistry. 1976 Jun 29;15(13):2863-8. doi: 10.1021/bi00658a025.