Anatomy Department, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Nigeria.
Human Anatomy Department, Federal University of Technology Akure, Akure, Nigeria.
Drug Chem Toxicol. 2022 Sep;45(5):1978-1985. doi: 10.1080/01480545.2021.1894721. Epub 2021 Mar 15.
The low Sulfur level, heavy metals and easy production rate of Bonny Light Crude Oil (BLCO) makes it one of Nigeria's most explored oil. This study investigated the memory impairments, embryotoxicity and cortico-hippocampal neurodegeneration induced by prenatal exposure to BLCO of pregnant Sprague-Dawley (S-D) rats. Twenty pregnant rats were divided into 4 groups (A-D) of 5 rats each. Group A received normal saline as . Group B-D received oral doses of BLCO at 0.73 ml/kg, 2 ml/kg and 3.8 ml/kg on pregnancy day 8-12.5 respectively. The pregnant rats were allowed to litter and nurse their pups. At 6 weeks postnatal life, twelve (12) selected young rats () were accessed for behavioral study (Y-maze) and then sacrificed for biochemical and histological analysis. The results showed spontaneous abortion, still births and significantly reduced number of live births in the high dose group of BLCO compared to control. Length of gestation was significantly increased in the high dose group when compared to the control. CAT levels reduced significantly with concomitant increase in 8-OHdG among BLCO treated groups compared to control. Spontaneous alteration and number of arm entries decreased in the BLCO groups in comparison to control. Histological observation showed reduced cellular size, chromatolysis and presence of extracellular senile plaques in the prefrontal cortex and mild histological changes in the hippocampus architecture in the BLCO treated groups compared to the control. BLCO is capable of inducing embryotoxicity, impair cognition and cortico-hippocampal neurodegeneration.
博尼轻质原油（Bonny Light Crude Oil，BLCO）含硫量低、重金属含量高且易于生产，是尼日利亚勘探最多的石油之一。本研究调查了产前暴露于 BLCO 对怀孕 Sprague-Dawley（S-D）大鼠的记忆障碍、胚胎毒性和皮质-海马神经退行性变的影响。20 只怀孕大鼠被分为 4 组（A-D），每组 5 只。A 组给予生理盐水作为对照。B-D 组在妊娠第 8-12.5 天分别给予 0.73ml/kg、2ml/kg 和 3.8ml/kg 的 BLCO 口服剂量。让怀孕的老鼠产仔并哺乳它们的幼崽。在出生后 6 周，选择 12 只（12 只）幼鼠进行行为研究（Y 迷宫），然后处死进行生化和组织学分析。结果显示，与对照组相比，BLCO 高剂量组出现自然流产、死产和活产数明显减少。与对照组相比，高剂量组的妊娠时间明显延长。与对照组相比，BLCO 处理组的 CAT 水平显著降低，同时 8-OHdG 水平显著升高。与对照组相比，BLCO 组的自发改变和臂进入次数减少。与对照组相比，BLCO 处理组的前额皮质细胞体积减小、色质溶解和细胞外老年斑的存在以及海马结构的轻度组织学变化。BLCO 能够诱导胚胎毒性、认知障碍和皮质-海马神经退行性变。
